Abstract
Overexpression of the 92 kDa type IV collagenase (MMP-9) contributes to cancer progression. However, to date, there are few known regulators of expression of this metalloproteinase. We employed an expression library comprising 500 000 cDNA clones to screen for novel regulators of MMP-9 expression. HT1080 cells were transiently co-transfected with an MMP-9 promoter-luciferase reporter and pools of the cDNA expression library. Positive-scoring pools were subdivided in secondary and tertiary screens, after which the regulatory cDNAs were identified by DNA sequencing. This brief review illustrates the utility of expression cloning in identifying specific regulators of MMP-9 expression.
Original language | English (US) |
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Pages (from-to) | 1135-1136 |
Number of pages | 2 |
Journal | Biochemical Society Transactions |
Volume | 33 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2005 |
Keywords
- 92 kDa type IV collagenase
- Cancer
- Expression cloning strategy
- HT1080 cells
- Matrix metalloproteinase-9 (MMP-9)
- Tumour progression
ASJC Scopus subject areas
- Biochemistry