Abstract
DNA-mediated gene transfer was used to introduce DNA from a methotrexate-resistant mouse fibroblast cell line into mouse bone marrow cells. This cell line contained a methotrexate-resistant dihydrofolate reductase, active at 10-4 M methotrexate, which was electrophoretically separable from the wild-type mouse enzyme. Transformed hematopoietic cells were returned to irradiated mice and selected in vivo by methotrexate administration. Some recipients of transformed marrow cells expressed the electrophoretically distinct, methotrexate-resistant dihydrofolate reductase in hematopoietic cells. These observations suggest that successful transformation of marrow stem cells to methotrexate resistance is accomplished by insertion of a dihydrofolate reductase gene coding for a mutant enzyme that is highly resistant to methotrexate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 55-67 |
| Number of pages | 13 |
| Journal | Somatic Cell Genetics |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1983 |
| Externally published | Yes |
ASJC Scopus subject areas
- Genetics
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