Abstract
IL-18 modulates immune functions by inducing IFN-γ production and promoting Th1 immune responses. In the present study, we amplified and cloned the sequence (582 bp) encoding full-length bovine IL-18 from PBMC stimulated with PHA. The nucleotide and the deduced amino acid sequence of Bos indicus IL-18 showed an identity of 86-98% compared with IL-18 sequences of other ruminants. The insert was subcloned into a pET 32a vector and expressed in Escherichia coli as a fusion protein and the matured protein was obtained by caspase I treatment. The specificity of these proteins was confirmed by western blotting. The biological activity of the purified protein was analyzed by its ability to induce IFN-γ production in PBMC measured by ELISA and qPCR.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 564-567 |
| Number of pages | 4 |
| Journal | Microbiology and Immunology |
| Volume | 54 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2010 |
| Externally published | Yes |
Keywords
- Biological activity
- Bos indicus
- Expression
- Interleukin (IL-18)
ASJC Scopus subject areas
- Microbiology
- Immunology
- Virology
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