TY - JOUR
T1 - Expression of cathepsin D in primary and metastatic human melanoma and dysplastic nevi
AU - Podhajcer, O. L.
AU - Bover, L.
AU - Bravo, A. I.
AU - Fernanda Ledda, M.
AU - Kairiyama, C.
AU - Calb, I.
AU - Guerra, L.
AU - Capony, F.
AU - Mordoh, J.
N1 - Funding Information:
This work was supported in part by a Special Grant from the Consejo Nacional de Investigaciones Cientijicas y Tecnicas (CONICET, Argentina) and the INSERM (France) . It was also supported by grants from the Fundacion para La Investigacion y Prevencion del Cancer (FUCA, Argentina), the Fundacion Sales (Argentina), and the International Centre for Genetic Engineering and Biotechnology (Italy). OLP, LB, MFL, LG, and JM are members of the CONICET, and FC is a member of the INSERM. We acknowledge Dr. Carlos Quintans, National Commission of Atomic Energy, for providing us with the nude mice.
PY - 1995
Y1 - 1995
N2 - High levels of cytosolic cathepsin D expression have been associated with poor prognosis in breast cancer node-negative patients. In this work, we provide evidence that three cell lines established from human metastatic melanomas - IIB-MEL-J, IIB-MEL-LES, and IIB-MEL-IAN - express high levels of procathepsin D mRNA. IIB-MEL-J cells secreted into the conditioned media about 30% of the newly synthesized protein, which was active at acidic pH. Melanoma tumors arising in nude mice after injection of the three different cell lines expressed high levels of procathepsin D mRNA. Moreover, 13 human metastatic melanomas expressed variable levels of procathepsin D mRNA. To study the possible association between cathepsin D expression and melanoma development, samples corresponding to 10 primary tumors, 11 metastatic melanomas, 10 dysplastic nevi, 27 nevocellular nevi, and normal melanocytes were studied by immunohistochemistry for cathepsin D-specific staining. We found that cathepsin D was expressed in all of the dysplastic nevi and primary and metastatic melanomas tested but in only 18% of nevocellular nevi (five of 27), whereas normal melanocytes showed no cathepsin D expression. The overall data indicate that cathepsin D is expressed at a high level by melanoma cells, and because of its expression in preneoplastic lesions, it may be associated with melanoma development.
AB - High levels of cytosolic cathepsin D expression have been associated with poor prognosis in breast cancer node-negative patients. In this work, we provide evidence that three cell lines established from human metastatic melanomas - IIB-MEL-J, IIB-MEL-LES, and IIB-MEL-IAN - express high levels of procathepsin D mRNA. IIB-MEL-J cells secreted into the conditioned media about 30% of the newly synthesized protein, which was active at acidic pH. Melanoma tumors arising in nude mice after injection of the three different cell lines expressed high levels of procathepsin D mRNA. Moreover, 13 human metastatic melanomas expressed variable levels of procathepsin D mRNA. To study the possible association between cathepsin D expression and melanoma development, samples corresponding to 10 primary tumors, 11 metastatic melanomas, 10 dysplastic nevi, 27 nevocellular nevi, and normal melanocytes were studied by immunohistochemistry for cathepsin D-specific staining. We found that cathepsin D was expressed in all of the dysplastic nevi and primary and metastatic melanomas tested but in only 18% of nevocellular nevi (five of 27), whereas normal melanocytes showed no cathepsin D expression. The overall data indicate that cathepsin D is expressed at a high level by melanoma cells, and because of its expression in preneoplastic lesions, it may be associated with melanoma development.
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U2 - 10.1111/1523-1747.ep12665371
DO - 10.1111/1523-1747.ep12665371
M3 - Article
C2 - 7860998
AN - SCOPUS:0028967470
SN - 0022-202X
VL - 104
SP - 340
EP - 344
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 3
ER -