Expression of semaphorin4C (Sema4C) in breast cancer, endometrial cancer and prostate cancer and its clinical significance

Ying Chen, Lei Zhang, Wenxin Liu, Gengxin Wang, Mingfu Wu, Ding Ma, Quan Hao

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    1 Scopus citations

    Abstract

    Objective: To evaluate the expression of Semaphorin4C (Sema4C) in breast cancer, endometrial cancer and prostate cancer tissues and seven cancer cell lines, including three human breast cancer cell lines (MDA-MB-435S, MDA-MB-231 and MCF-7), two human prostate cancer cell lines (PC-3M-1E8 and PC-3M-2B4), and two human endometrial cancer cell lines (AN3CA and HEC-1-B) and to determine its clinical significance. Methods: Highly sensitive SP immunohistochemistry was used to detect the expression of Sema4C in 45 samples of invasive ductal breast cancer, 42 samples of endometrial cancer, and 49 samples of prostate cancer. Western blot was used to detect the expression of Sema4C in the seven cancer cell lines. The subcellular localization of Sema4C was observed by cell immunofluorescence chemistry. Results: In human breast and endometrial cancer tissues, a high expression of Sema4C was observed in cases with lymph node metastasis, with a significant difference (P<0.05). In prostate cancer tissues, a high expression of Sema4C was observed in cases with Gleason 8-10 (P<0.05). Western blot revealed that the expression of Sema4C was higher in high-metastatic cell lines (MDA-MB-435S, MDA-MB-231, PC-3M-1E8 and AN3CA cells) than in low-metastatic cell lines (MCF-7, PC-3M-2B4 and HEC-1-B cells). The Sema4C protein was mainly expressed in the cytoplasm and membrane. Conclusion: The expression of Sema4C is universal in breast cancer, endometrial cancer and prostate cancer tissues and cell lines. Sema4C may play a role in the metastasis of the three cancers.

    Original languageEnglish (US)
    Pages (from-to)507-511
    Number of pages5
    JournalChinese Journal of Clinical Oncology
    Volume37
    Issue number9
    DOIs
    StatePublished - May 15 2010

    Keywords

    • Cancer
    • Metastasis
    • Semaphorin4C

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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