Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells

Hima V. Vangapandu, Varsha V Gandhi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Extracellular flux assays are conducted using seahorse XF96 analyzer. They are used to calculate oxygen consumption rate which is to determine mitochondrial oxidative phosphorylation and extracellular acidification rate which is a measure of glycolysis. Collectively, these assays are used to assess the metabolic phenotype of a cell. Up to four drugs can be loaded and tested in the XF cartridges used in the assay and their effect on cells could be determined. While adherent cell lines are easy to use for this assay, suspension cultures or primary cells are difficult to use. In the following sections, we describe the methodology for this assay for CLL cells in suspension cultures and CLL-stroma cocultures.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-128
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1881
ISSN (Print)1064-3745

Fingerprint

Oxidative Phosphorylation
Glycolysis
B-Cell Chronic Lymphocytic Leukemia
Suspensions
Smegmamorpha
Primary Cell Culture
Coculture Techniques
Oxygen Consumption
Phenotype
Cell Line
Pharmaceutical Preparations

Keywords

  • ECAR
  • Extracellular flux
  • Metabolism
  • OCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Vangapandu, H. V., & Gandhi, V. V. (2019). Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells. In Methods in Molecular Biology (pp. 121-128). (Methods in Molecular Biology; Vol. 1881). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8876-1_10

Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells. / Vangapandu, Hima V.; Gandhi, Varsha V.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 121-128 (Methods in Molecular Biology; Vol. 1881).

Research output: Chapter in Book/Report/Conference proceedingChapter

Vangapandu, HV & Gandhi, VV 2019, Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1881, Humana Press Inc., pp. 121-128. https://doi.org/10.1007/978-1-4939-8876-1_10
Vangapandu HV, Gandhi VV. Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 121-128. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8876-1_10
Vangapandu, Hima V. ; Gandhi, Varsha V. / Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 121-128 (Methods in Molecular Biology).
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