Fraction B From Catfish Epidermal Secretions Kills Pancreatic Cancer Cells, Inhibits CD44 Expression and Stemness, and Alters Cancer Cell Metabolism

Jassim M. Al-Hassan; Daoyan Wei; Sharmistha Chakraborty; Tara Conway; Patrea Rhea; Bo Wei; Megan Tran; Mihai Gagea; Mohammad Afzal; Sosamma Oommen; Divya Nair; Bincy M. Paul; Peiying Yang

doi:10.3389/fphar.2021.659590

Fraction B From Catfish Epidermal Secretions Kills Pancreatic Cancer Cells, Inhibits CD44 Expression and Stemness, and Alters Cancer Cell Metabolism

Jassim M. Al-Hassan, Daoyan Wei, Sharmistha Chakraborty, Tara Conway, Patrea Rhea, Bo Wei, Megan Tran, Mihai Gagea, Mohammad Afzal, Sosamma Oommen, Divya Nair, Bincy M. Paul, Peiying Yang

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer related death in western countries. The successful treatment of PDAC remains limited. We investigated the effect of Fraction B, which is a fraction purified from catfish (Arius bilineatus, Val.) skin secretions containing proteins and lipids, on PDAC biology both in-vivo and in-vitro. We report here that Fraction B potently suppressed the proliferation of both human and mouse pancreatic cancer cells in vitro and significantly reduced the growth of their relevant xenograft (Panc02) and orthotopic tumors (human Panc-1 cells) (p < 0.05). The Reverse Phase Protein Array (RPPA) data obtained from the tumor tissues derived from orthotopic tumor bearing mice treated with Fraction B showed that Fraction B altered the cancer stem cells related pathways and regulated glucose and glutamine metabolism. The down-regulation of the cancer stem cell marker CD44 expression was further confirmed in Panc-1 cells. CBC and blood chemistry analyses showed no systemic toxicity in Fraction B treated Panc-1 tumor bearing mice compared to that of control group. Our data support that Fraction B is a potential candidate for PDAC treatment.

Original language	English (US)
Article number	659590
Journal	Frontiers in Pharmacology
Volume	12
DOIs	https://doi.org/10.3389/fphar.2021.659590
State	Published - Jul 19 2021

Keywords

apoptosis
cancer metabolism
cancer stemness
catfish
Fraction B
pancreatic cancer

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)

MD Anderson CCSG core facilities

Functional Proteomics Reverse Phase Protein Array Core
Bioinformatics Shared Resource
Research Animal Support Facility
Small Animal Imaging Facility

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10.3389/fphar.2021.659590

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Al-Hassan, J. M., Wei, D., Chakraborty, S., Conway, T., Rhea, P., Wei, B., Tran, M., Gagea, M., Afzal, M., Oommen, S., Nair, D., Paul, B. M., & Yang, P. (2021). Fraction B From Catfish Epidermal Secretions Kills Pancreatic Cancer Cells, Inhibits CD44 Expression and Stemness, and Alters Cancer Cell Metabolism. Frontiers in Pharmacology, 12, Article 659590. https://doi.org/10.3389/fphar.2021.659590

Al-Hassan, JM, Wei, D , Chakraborty, S, Conway, T, Rhea, P, Wei, B, Tran, M, Gagea, M, Afzal, M, Oommen, S, Nair, D, Paul, BM & Yang, P 2021, 'Fraction B From Catfish Epidermal Secretions Kills Pancreatic Cancer Cells, Inhibits CD44 Expression and Stemness, and Alters Cancer Cell Metabolism', Frontiers in Pharmacology, vol. 12, 659590. https://doi.org/10.3389/fphar.2021.659590

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title = "Fraction B From Catfish Epidermal Secretions Kills Pancreatic Cancer Cells, Inhibits CD44 Expression and Stemness, and Alters Cancer Cell Metabolism",

abstract = "Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer related death in western countries. The successful treatment of PDAC remains limited. We investigated the effect of Fraction B, which is a fraction purified from catfish (Arius bilineatus, Val.) skin secretions containing proteins and lipids, on PDAC biology both in-vivo and in-vitro. We report here that Fraction B potently suppressed the proliferation of both human and mouse pancreatic cancer cells in vitro and significantly reduced the growth of their relevant xenograft (Panc02) and orthotopic tumors (human Panc-1 cells) (p < 0.05). The Reverse Phase Protein Array (RPPA) data obtained from the tumor tissues derived from orthotopic tumor bearing mice treated with Fraction B showed that Fraction B altered the cancer stem cells related pathways and regulated glucose and glutamine metabolism. The down-regulation of the cancer stem cell marker CD44 expression was further confirmed in Panc-1 cells. CBC and blood chemistry analyses showed no systemic toxicity in Fraction B treated Panc-1 tumor bearing mice compared to that of control group. Our data support that Fraction B is a potential candidate for PDAC treatment.",

keywords = "apoptosis, cancer metabolism, cancer stemness, catfish, Fraction B, pancreatic cancer",

author = "Al-Hassan, {Jassim M.} and Daoyan Wei and Sharmistha Chakraborty and Tara Conway and Patrea Rhea and Bo Wei and Megan Tran and Mihai Gagea and Mohammad Afzal and Sosamma Oommen and Divya Nair and Paul, {Bincy M.} and Peiying Yang",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Al-Hassan, Wei, Chakraborty, Conway, Rhea, Wei, Tran, Gagea, Afzal, Oommen, Nair, Paul and Yang.",

year = "2021",

month = jul,

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doi = "10.3389/fphar.2021.659590",

language = "English (US)",

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journal = "Frontiers in Pharmacology",

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T1 - Fraction B From Catfish Epidermal Secretions Kills Pancreatic Cancer Cells, Inhibits CD44 Expression and Stemness, and Alters Cancer Cell Metabolism

AU - Al-Hassan, Jassim M.

AU - Wei, Daoyan

AU - Chakraborty, Sharmistha

AU - Conway, Tara

AU - Rhea, Patrea

AU - Wei, Bo

AU - Tran, Megan

AU - Gagea, Mihai

AU - Afzal, Mohammad

AU - Oommen, Sosamma

AU - Nair, Divya

AU - Paul, Bincy M.

AU - Yang, Peiying

PY - 2021/7/19

Y1 - 2021/7/19

N2 - Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer related death in western countries. The successful treatment of PDAC remains limited. We investigated the effect of Fraction B, which is a fraction purified from catfish (Arius bilineatus, Val.) skin secretions containing proteins and lipids, on PDAC biology both in-vivo and in-vitro. We report here that Fraction B potently suppressed the proliferation of both human and mouse pancreatic cancer cells in vitro and significantly reduced the growth of their relevant xenograft (Panc02) and orthotopic tumors (human Panc-1 cells) (p < 0.05). The Reverse Phase Protein Array (RPPA) data obtained from the tumor tissues derived from orthotopic tumor bearing mice treated with Fraction B showed that Fraction B altered the cancer stem cells related pathways and regulated glucose and glutamine metabolism. The down-regulation of the cancer stem cell marker CD44 expression was further confirmed in Panc-1 cells. CBC and blood chemistry analyses showed no systemic toxicity in Fraction B treated Panc-1 tumor bearing mice compared to that of control group. Our data support that Fraction B is a potential candidate for PDAC treatment.

AB - Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer related death in western countries. The successful treatment of PDAC remains limited. We investigated the effect of Fraction B, which is a fraction purified from catfish (Arius bilineatus, Val.) skin secretions containing proteins and lipids, on PDAC biology both in-vivo and in-vitro. We report here that Fraction B potently suppressed the proliferation of both human and mouse pancreatic cancer cells in vitro and significantly reduced the growth of their relevant xenograft (Panc02) and orthotopic tumors (human Panc-1 cells) (p < 0.05). The Reverse Phase Protein Array (RPPA) data obtained from the tumor tissues derived from orthotopic tumor bearing mice treated with Fraction B showed that Fraction B altered the cancer stem cells related pathways and regulated glucose and glutamine metabolism. The down-regulation of the cancer stem cell marker CD44 expression was further confirmed in Panc-1 cells. CBC and blood chemistry analyses showed no systemic toxicity in Fraction B treated Panc-1 tumor bearing mice compared to that of control group. Our data support that Fraction B is a potential candidate for PDAC treatment.

KW - apoptosis

KW - cancer metabolism

KW - cancer stemness

KW - catfish

KW - Fraction B

KW - pancreatic cancer

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Fraction B From Catfish Epidermal Secretions Kills Pancreatic Cancer Cells, Inhibits CD44 Expression and Stemness, and Alters Cancer Cell Metabolism

Abstract

Keywords

ASJC Scopus subject areas

MD Anderson CCSG core facilities

Access to Document

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