TY - JOUR
T1 - From pathways to networks
T2 - Connecting dots by establishing protein-protein interaction networks in signaling pathways using affinity purification and mass spectrometry
AU - Li, Xu
AU - Wang, Wenqi
AU - Chen, Junjie
N1 - Publisher Copyright:
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Signal transductions are the basis of biological activities in all living organisms. Studying the signaling pathways, especially under physiological conditions, has become one of the most important facets of modern biological research. During the last decade, MS has been used extensively in biological research and is proven to be effective in addressing important biological questions. Here, we review the current progress in the understanding of signaling networks using MS approaches. We will focus on studies of protein-protein interactions that use affinity purification followed by MS approach. We discuss obstacles to affinity purification, data processing, functional validation, and identification of transient interactions and provide potential solutions for pathway-specific proteomics analysis, which we hope one day will lead to a comprehensive understanding of signaling networks in humans.
AB - Signal transductions are the basis of biological activities in all living organisms. Studying the signaling pathways, especially under physiological conditions, has become one of the most important facets of modern biological research. During the last decade, MS has been used extensively in biological research and is proven to be effective in addressing important biological questions. Here, we review the current progress in the understanding of signaling networks using MS approaches. We will focus on studies of protein-protein interactions that use affinity purification followed by MS approach. We discuss obstacles to affinity purification, data processing, functional validation, and identification of transient interactions and provide potential solutions for pathway-specific proteomics analysis, which we hope one day will lead to a comprehensive understanding of signaling networks in humans.
KW - Affinity purification
KW - Animal proteomics
KW - Hippo signaling
KW - Mass spectrometry
KW - Protein-protein interaction
KW - Signaling pathways
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U2 - 10.1002/pmic.201400147
DO - 10.1002/pmic.201400147
M3 - Review article
C2 - 25137225
AN - SCOPUS:84921273143
SN - 1615-9853
VL - 15
SP - 188
EP - 202
JO - Proteomics
JF - Proteomics
IS - 2-3
ER -