Functional optical coherence tomography for high-resolution mapping of cilia beat frequency in the mouse oviduct in vivo

Shang Wang, Jason C. Burton, Richard R. Behringer, Irina V. Larina

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Since mouse is a superior model for genetic analysis of human disorders, reproductive studies in mice have significant implications on further understanding of fertility and infertility in humans. Fertilized oocytes are transported through the reproductive tract by motile cilia lining the lumen of the oviduct as well as by oviduct contractions. While the role of cilia is well recognized, ciliary dynamics in the oviduct is not well understood, largely owing to the lack of live imaging approaches. Here, we report in vivo micro-scale mapping of cilia and cilia beat frequency (CBF) in the mouse oviduct using optical coherence tomography (OCT). This functional imaging method is based on spectral analysis of the OCT speckle variations produced by the beat of cilia in the oviduct, which does not require exogenous contrast agents. Animal procedures similar to the ones used for production of transgenic mice are utilized to expose the reproductive organs for imaging in anesthetized females. In this paper, we first present in vivo structural imaging of the mouse oviduct capturing the oocyte and the preimplantation embryo and then show the result of depth-resolved high-resolution CBF mapping in the ampulla of the live mouse. These data indicate that this structural and functional OCT imaging approach can be a useful tool for a variety of live investigations of mammalian reproduction and infertility.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics XII
EditorsAndreas Mandelis, Bernard Choi, Brian J. F. Wong, Justus F. Ilgner, Laura Marcu, Melissa C. Skala, Nikiforos Kollias, Haishan Zeng, Hyun Wook Kang, Guillermo J. Tearney, Kenton W. Gregory, Paul J. Campagnola
ISBN (Electronic)9781628419245
StatePublished - 2016
EventPhotonic Therapeutics and Diagnostics XII - San Francisco, United States
Duration: Feb 13 2016Feb 14 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherPhotonic Therapeutics and Diagnostics XII
Country/TerritoryUnited States
CitySan Francisco


  • cilia beat frequency
  • cumulus cells
  • functional imaging
  • in vivo
  • infertility
  • mammalian reproduction
  • mouse oviduct
  • oocyte
  • optical coherence tomography
  • speckles

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


Dive into the research topics of 'Functional optical coherence tomography for high-resolution mapping of cilia beat frequency in the mouse oviduct in vivo'. Together they form a unique fingerprint.

Cite this