Gain of the 3q26 region in cervicovaginal liquid-based pap preparations is associated with squamous intraepithelial lesions and squamous cell carcinoma

Nancy P Caraway, Abha Khanna, Marilyn Dawlett, Ming Guo, Nina Guo, E. Lin, Ruth L Katz

Research output: Contribution to journalArticle

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Abstract

Background: Chromosomal aberrations have been documented in cervical carcinomas, especially chromosome 3q. The human telomerase RNA gene (hTERC) is located in the chromosome 3q26 region, and its product, telomerase, is involved in the maintenance of chromosome length and stability. Upregulation of telomerase is in general associated with tumorigenesis. In this study, cervicovaginal specimens were analyzed by fluorescence in situ hybridization (FISH) for gain of chromosome 3q26 containing hTERC, and FISH findings were compared with the cytologic and histologic diagnoses. Methods: Slides prepared from 66 liquid-based preparations from cervical specimens with cytologic diagnoses of negative for squamous intraepithelial lesion or malignancy (NILM, n = 4), atypical squamous cells of undetermined significance (ASC-US, n = 15), low-grade squamous intraepithelial lesion (LSIL, n = 20), high-grade squamous intraepithelial lesion (HSIL, n = 24), or cervical squamous cell carcinoma (SCCA, n = 3) were analyzed for aberrations of 3q26 using a commercially available two-color FISH probe. The results of the cytologic analysis and those of concurrent or subsequent biopsies, when available, were compared with the FISH-detected 3q26 abnormalities. The Wilcoxon rank-sum test was used to assess associations between 3q26 gains and diagnoses. Results: Gain of 3q26 was significantly associated with the cytologic diagnosis (p < 0.0001). Patients with HSIL or SCCA cytology diagnoses had significantly higher percentages of cells with 3q26 gain than did patients with NILM or ASC-US cytologic diagnoses. Conclusions: FISH can be performed on cervicovaginal liquid-based preparations to detect gain of 3q26. Gain of 3q26 is associated with HSIL and SCCA. This test may be an adjunct to cytology screening, especially high-risk patients.

Original languageEnglish (US)
Pages (from-to)37-42
Number of pages6
JournalGynecologic oncology
Volume110
Issue number1
DOIs
StatePublished - Jul 1 2008

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Squamous Cell Carcinoma
Fluorescence In Situ Hybridization
Chromosomes
Telomerase
Nonparametric Statistics
Cell Biology
Chromosomal Instability
Chromosome Aberrations
Squamous Intraepithelial Lesions of the Cervix
Carcinogenesis
Up-Regulation
Color
Maintenance
Carcinoma
Biopsy
Genes
Atypical Squamous Cells of the Cervix
Neoplasms

Keywords

  • Cervical cancer
  • Cervical intraepithelial lesion
  • Chromosomal abnormalities
  • Chromosome 3q26
  • FISH
  • Liquid-based cytology
  • Squamous intraepithelial lesion
  • Telomerase RNA gene
  • Telomerase gene
  • hTERC

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

Cite this

Gain of the 3q26 region in cervicovaginal liquid-based pap preparations is associated with squamous intraepithelial lesions and squamous cell carcinoma. / Caraway, Nancy P; Khanna, Abha; Dawlett, Marilyn; Guo, Ming; Guo, Nina; Lin, E.; Katz, Ruth L.

In: Gynecologic oncology, Vol. 110, No. 1, 01.07.2008, p. 37-42.

Research output: Contribution to journalArticle

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title = "Gain of the 3q26 region in cervicovaginal liquid-based pap preparations is associated with squamous intraepithelial lesions and squamous cell carcinoma",
abstract = "Background: Chromosomal aberrations have been documented in cervical carcinomas, especially chromosome 3q. The human telomerase RNA gene (hTERC) is located in the chromosome 3q26 region, and its product, telomerase, is involved in the maintenance of chromosome length and stability. Upregulation of telomerase is in general associated with tumorigenesis. In this study, cervicovaginal specimens were analyzed by fluorescence in situ hybridization (FISH) for gain of chromosome 3q26 containing hTERC, and FISH findings were compared with the cytologic and histologic diagnoses. Methods: Slides prepared from 66 liquid-based preparations from cervical specimens with cytologic diagnoses of negative for squamous intraepithelial lesion or malignancy (NILM, n = 4), atypical squamous cells of undetermined significance (ASC-US, n = 15), low-grade squamous intraepithelial lesion (LSIL, n = 20), high-grade squamous intraepithelial lesion (HSIL, n = 24), or cervical squamous cell carcinoma (SCCA, n = 3) were analyzed for aberrations of 3q26 using a commercially available two-color FISH probe. The results of the cytologic analysis and those of concurrent or subsequent biopsies, when available, were compared with the FISH-detected 3q26 abnormalities. The Wilcoxon rank-sum test was used to assess associations between 3q26 gains and diagnoses. Results: Gain of 3q26 was significantly associated with the cytologic diagnosis (p < 0.0001). Patients with HSIL or SCCA cytology diagnoses had significantly higher percentages of cells with 3q26 gain than did patients with NILM or ASC-US cytologic diagnoses. Conclusions: FISH can be performed on cervicovaginal liquid-based preparations to detect gain of 3q26. Gain of 3q26 is associated with HSIL and SCCA. This test may be an adjunct to cytology screening, especially high-risk patients.",
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T1 - Gain of the 3q26 region in cervicovaginal liquid-based pap preparations is associated with squamous intraepithelial lesions and squamous cell carcinoma

AU - Caraway, Nancy P

AU - Khanna, Abha

AU - Dawlett, Marilyn

AU - Guo, Ming

AU - Guo, Nina

AU - Lin, E.

AU - Katz, Ruth L

PY - 2008/7/1

Y1 - 2008/7/1

N2 - Background: Chromosomal aberrations have been documented in cervical carcinomas, especially chromosome 3q. The human telomerase RNA gene (hTERC) is located in the chromosome 3q26 region, and its product, telomerase, is involved in the maintenance of chromosome length and stability. Upregulation of telomerase is in general associated with tumorigenesis. In this study, cervicovaginal specimens were analyzed by fluorescence in situ hybridization (FISH) for gain of chromosome 3q26 containing hTERC, and FISH findings were compared with the cytologic and histologic diagnoses. Methods: Slides prepared from 66 liquid-based preparations from cervical specimens with cytologic diagnoses of negative for squamous intraepithelial lesion or malignancy (NILM, n = 4), atypical squamous cells of undetermined significance (ASC-US, n = 15), low-grade squamous intraepithelial lesion (LSIL, n = 20), high-grade squamous intraepithelial lesion (HSIL, n = 24), or cervical squamous cell carcinoma (SCCA, n = 3) were analyzed for aberrations of 3q26 using a commercially available two-color FISH probe. The results of the cytologic analysis and those of concurrent or subsequent biopsies, when available, were compared with the FISH-detected 3q26 abnormalities. The Wilcoxon rank-sum test was used to assess associations between 3q26 gains and diagnoses. Results: Gain of 3q26 was significantly associated with the cytologic diagnosis (p < 0.0001). Patients with HSIL or SCCA cytology diagnoses had significantly higher percentages of cells with 3q26 gain than did patients with NILM or ASC-US cytologic diagnoses. Conclusions: FISH can be performed on cervicovaginal liquid-based preparations to detect gain of 3q26. Gain of 3q26 is associated with HSIL and SCCA. This test may be an adjunct to cytology screening, especially high-risk patients.

AB - Background: Chromosomal aberrations have been documented in cervical carcinomas, especially chromosome 3q. The human telomerase RNA gene (hTERC) is located in the chromosome 3q26 region, and its product, telomerase, is involved in the maintenance of chromosome length and stability. Upregulation of telomerase is in general associated with tumorigenesis. In this study, cervicovaginal specimens were analyzed by fluorescence in situ hybridization (FISH) for gain of chromosome 3q26 containing hTERC, and FISH findings were compared with the cytologic and histologic diagnoses. Methods: Slides prepared from 66 liquid-based preparations from cervical specimens with cytologic diagnoses of negative for squamous intraepithelial lesion or malignancy (NILM, n = 4), atypical squamous cells of undetermined significance (ASC-US, n = 15), low-grade squamous intraepithelial lesion (LSIL, n = 20), high-grade squamous intraepithelial lesion (HSIL, n = 24), or cervical squamous cell carcinoma (SCCA, n = 3) were analyzed for aberrations of 3q26 using a commercially available two-color FISH probe. The results of the cytologic analysis and those of concurrent or subsequent biopsies, when available, were compared with the FISH-detected 3q26 abnormalities. The Wilcoxon rank-sum test was used to assess associations between 3q26 gains and diagnoses. Results: Gain of 3q26 was significantly associated with the cytologic diagnosis (p < 0.0001). Patients with HSIL or SCCA cytology diagnoses had significantly higher percentages of cells with 3q26 gain than did patients with NILM or ASC-US cytologic diagnoses. Conclusions: FISH can be performed on cervicovaginal liquid-based preparations to detect gain of 3q26. Gain of 3q26 is associated with HSIL and SCCA. This test may be an adjunct to cytology screening, especially high-risk patients.

KW - Cervical cancer

KW - Cervical intraepithelial lesion

KW - Chromosomal abnormalities

KW - Chromosome 3q26

KW - FISH

KW - Liquid-based cytology

KW - Squamous intraepithelial lesion

KW - Telomerase RNA gene

KW - Telomerase gene

KW - hTERC

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