Gcn5 and SAGA Regulate Shelterin Protein Turnover and Telomere Maintenance

Boyko S. Atanassov, Yvonne A. Evrard, Asha S. Multani, Zhijing Zhang, László Tora, Didier Devys, Sandy Chang, Sharon Y.R. Dent

Research output: Contribution to journalArticlepeer-review

139 Scopus citations

Abstract

Histone acetyltransferases (HATs) play important roles in gene regulation and DNA repair by influencing the accessibility of chromatin to transcription factors and repair proteins. Here, we show that deletion of Gcn5 leads to telomere dysfunction in mouse and human cells. Biochemical studies reveal that depletion of Gcn5 or ubiquitin-specific protease 22 (Usp22), which is another bona fide component of the Gcn5-containing SAGA complex, increases ubiquitination and turnover of TRF1, a primary component of the telomeric shelterin complex. Inhibition of the proteasome or overexpression of USP22 opposes this effect. The USP22 deubiquitinating module requires association with SAGA complexes for activity, and we find that depletion of Gcn5 compromises this association in mammalian cells. Thus, our results indicate that Gcn5 regulates TRF1 levels through effects on Usp22 activity and SAGA integrity.

Original languageEnglish (US)
Pages (from-to)352-364
Number of pages13
JournalMolecular cell
Volume35
Issue number3
DOIs
StatePublished - Aug 14 2009

Keywords

  • DNA
  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

MD Anderson CCSG core facilities

  • Advanced Technology Genomics Core
  • Research Animal Support Facility

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