Gene expression profile of Dclk1 + cells in intestinal tumors

Yuichi Yamaga, Akihisa Fukuda, Yuki Nakanishi, Norihiro Goto, Yoshihide Matsumoto, Takuto Yoshioka, Takahisa Maruno, Tsutomu Chiba, Hiroshi Seno

    Research output: Contribution to journalArticle

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    Abstract

    Background: Accumulating evidence has shown the existence of tumor stem cells with therapeutic potential. Previously, we reported that doublecortin like kinase 1 (Dclk1) marks tumor stem cells but not normal stem cells in the intestine of Apc Min/+ mice, and that Dclk1- and Lgr5-double positive tumor cells are the tumor stem cells of intestinal tumors. Aim: To investigate molecules highly expressed in the Dclk1 + normal intestinal and Dclk1 + tumor cells in Apc Min/+ mice. Methods: We used microarray analyses to examine the gene expression profile of Dclk1 + cells in both mouse normal intestinal epithelium and Apc Min/+ mouse intestinal tumors. We also performed immunofluorescence analyses. Results: Genes related to microtubules and the actin cytoskeleton (e.g., Rac2), and members of the Src family kinases (i.e., Hck, Lyn, Csk, and Ptpn6) were highly expressed in both Dclk1 + normal intestinal and Dclk1 + tumor cells. Phosphorylated Hck and phosphorylated Lyn were expressed in Lgr5 + cells in the intestinal tumors of Lgr5 EGFP-IRES-CreERT2/+ ; Apc Min/+ mice. Conclusion: We revealed factors that are highly expressed in Dclk1 + intestinal tumor cells, which may help to develop cancer stem cell-targeted therapy in future.

    Original languageEnglish (US)
    Pages (from-to)1353-1361
    Number of pages9
    JournalDigestive and Liver Disease
    Volume50
    Issue number12
    DOIs
    StatePublished - Dec 2018

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    Keywords

    • Colorectal cancer
    • Microarray analysis
    • Tumor stem cells

    ASJC Scopus subject areas

    • Hepatology
    • Gastroenterology

    Cite this

    Yamaga, Y., Fukuda, A., Nakanishi, Y., Goto, N., Matsumoto, Y., Yoshioka, T., Maruno, T., Chiba, T., & Seno, H. (2018). Gene expression profile of Dclk1 + cells in intestinal tumors Digestive and Liver Disease, 50(12), 1353-1361. https://doi.org/10.1016/j.dld.2018.06.011