Gene expression profiling of synovial sarcoma: Distinct signature of poorly differentiated type

Robert Nakayama, Sachiyo Mitani, Takeshi Nakagawa, Tadashi Hasegawa, Akira Kawai, Hideo Morioka, Hiroo Yabe, Yoshiaki Toyama, Akira Ogose, Junya Toguchida, Tomitaka Nakayama, Teruhiko Yoshida, Hitoshi Ichikawa

    Research output: Contribution to journalArticle

    28 Scopus citations

    Abstract

    Poorly differentiated type synovial sarcoma (PDSS) is a variant of synovial sarcoma characterized by predominantly round or short-spindled cell morphology. Although accumulating evidence from clinicopathologic studies suggests a strong association between this variant of synovial sarcoma and poor prognosis, little has been reported on the molecular basis of PDSS. To gain insights into the mechanism(s) that underlie the emergence of PDSS, we analyzed the gene expression profiles of 34 synovial sarcoma clinical samples, including 5 cases of PDSS, using an oligonucleotide microarray. In an unsupervised analysis, the 34 samples fell into 3 groups that correlate closely with histologic subtypes: monophasic, biphasic, and poorly differentiated types. PDSS was characterized by down-regulation of genes associated with neuronal and skeletal development and cell adhesion. Moreover, upregulation of genes on a specific chromosomal locus, 8q21.11, was identified. This locus-specific transcriptional activation in PDSS was confirmed by reverse transcriptase-PCR analysis of 9 additional synovial sarcoma samples. Our results indicate that PDSS tumors constitute a distinct group based on expression profiles.

    Original languageEnglish (US)
    Pages (from-to)1599-1607
    Number of pages9
    JournalAmerican Journal of Surgical Pathology
    Volume34
    Issue number11
    DOIs
    StatePublished - Jan 1 2010

      Fingerprint

    Keywords

    • gene expression profiling
    • poorly differentiated type
    • synovial sarcoma

    ASJC Scopus subject areas

    • Anatomy
    • Surgery
    • Pathology and Forensic Medicine

    Cite this

    Nakayama, R., Mitani, S., Nakagawa, T., Hasegawa, T., Kawai, A., Morioka, H., Yabe, H., Toyama, Y., Ogose, A., Toguchida, J., Nakayama, T., Yoshida, T., & Ichikawa, H. (2010). Gene expression profiling of synovial sarcoma: Distinct signature of poorly differentiated type. American Journal of Surgical Pathology, 34(11), 1599-1607. https://doi.org/10.1097/PAS.0b013e3181f7ce2c