Genetic mapping of restriction landmark genomic scanning loci in the mouse

Hisato Okuizumi, Yasushi Okazaki, Tomoya Ohsumi, Yoshihide Hayashizaki, Christoph Plass, Verne M. Chapman

    Research output: Contribution to journalArticle

    14 Scopus citations

    Abstract

    Restriction landmark genomic scanning (RLGS) was originally proposed as a high‐speed method for surveying a large number of restriction landmarks in genomic DNA. The effort to apply this method to genetic analysis has been made, resulting in developing the new approach for the rapid construction of the genetic map of complex mammalian genomes (RLGS spot mapping). Especially, the use of NotI as the restriction landmark for genetic studies suggests that there is a high probability that a significant number of these RLGS loci will be associated with CpG islands of functional genes. Moreover, it is possible to use the RLGS spot mapping to analyze genetic map‐poor species very rapidly for linkage of recessive mutations or segregating traits, because it does not rely upon cloned probes or sequences. In this paper, we summarize the progress that has been made in the practical application of the RLGS method to genetic analysis using congenic strains, recombinant inbred (RI) strains, and in interspecific backcrosses of mice.

    Original languageEnglish (US)
    Pages (from-to)233-240
    Number of pages8
    JournalElectrophoresis
    Volume16
    Issue number1
    DOIs
    StatePublished - Jan 1 1995

    Keywords

    • Genetic mapping
    • Mouse
    • Restriction landmark genomic scanning

    ASJC Scopus subject areas

    • Biochemistry
    • Clinical Biochemistry

    Fingerprint Dive into the research topics of 'Genetic mapping of restriction landmark genomic scanning loci in the mouse'. Together they form a unique fingerprint.

  • Cite this

    Okuizumi, H., Okazaki, Y., Ohsumi, T., Hayashizaki, Y., Plass, C., & Chapman, V. M. (1995). Genetic mapping of restriction landmark genomic scanning loci in the mouse. Electrophoresis, 16(1), 233-240. https://doi.org/10.1002/elps.1150160139