TY - JOUR
T1 - Genetic Variation of SARS Coronavirus in Beijing Hospital
AU - Xu, Dongping
AU - Zhang, Zheng
AU - Chu, Fuliang
AU - Li, Yonggang
AU - Jin, Lei
AU - Zhang, Lingxia
AU - Gao, George F.
AU - Wang, Fu Sheng
PY - 2004/5
Y1 - 2004/5
N2 - To characterize genetic variation of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) transmitted in the Beijing area during the epidemic outbreak of 2003, we sequenced 29 full-length S genes of SARS-CoV from 20 hospitalized SARS patients on our unit, the Beijing 302 Hospital. Viral RNA templates for the S-gene amplification were directly extracted from raw clinical samples, including plasma, throat swab, sputum, and stool, during the course of the epidemic in the Beijing area. We used a TA-cloning assay with direct analysis of nested reverse transcription-polymerase chain reaction products in sequence. One hundred thirteen sequence variations with nine recurrent variant sites were identified in analyzed S-gene sequences compared with the BJ01 strain of SARS-CoV. Among them, eight variant sites were, we think, the first documented. Our findings demonstrate the coexistence of S-gene sequences with and without substitutions (compared to BJ01) in samples analyzed from some patients.
AB - To characterize genetic variation of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) transmitted in the Beijing area during the epidemic outbreak of 2003, we sequenced 29 full-length S genes of SARS-CoV from 20 hospitalized SARS patients on our unit, the Beijing 302 Hospital. Viral RNA templates for the S-gene amplification were directly extracted from raw clinical samples, including plasma, throat swab, sputum, and stool, during the course of the epidemic in the Beijing area. We used a TA-cloning assay with direct analysis of nested reverse transcription-polymerase chain reaction products in sequence. One hundred thirteen sequence variations with nine recurrent variant sites were identified in analyzed S-gene sequences compared with the BJ01 strain of SARS-CoV. Among them, eight variant sites were, we think, the first documented. Our findings demonstrate the coexistence of S-gene sequences with and without substitutions (compared to BJ01) in samples analyzed from some patients.
UR - http://www.scopus.com/inward/record.url?scp=2142709586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2142709586&partnerID=8YFLogxK
U2 - 10.3201/eid1005.030875
DO - 10.3201/eid1005.030875
M3 - Article
C2 - 15200810
AN - SCOPUS:2142709586
SN - 1080-6040
VL - 10
SP - 789
EP - 794
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 5
ER -