Genomic organization, chromosomal localization, and expression of the murine RAB3D gene

Roberto Adachi, Rupesh Nigam, Michael J. Tuvim, Francesco Demayo, Burton F. Dickey

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Rab proteins, members of the Ras superfamily of small GTPases, play regulatory roles in intercompartmental vesicular transport. Each step of traffic seems to require the participation of at least one distinct Rab, with the Rab3 subfamily involved in stimulated exocytosis. We report our studies on the murine rab3D gene, one of the four mammalian Rab3 isoforms. We located this gene on chromosome 13, region A2-3. The rab3D gene consists of 5 exons spanning 10.6 kb, and the structural gene is contained in exons 2 through 5 with one canonical GTP-binding motif in each exon. Organization of the rab3D gene is identical to that of rab3A but different from other rab genes. Alternative poly-A+ signals in the 3' untranslated region account for the identities of multiple transcripts detected by Northern blot analysis. Rab3D is expressed in all tissues studied, predominantly in heart, lung, and liver, and binding sites for multiple transcription factors are found in the TATA-less promoter region. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)877-883
Number of pages7
JournalBiochemical and biophysical research communications
Volume273
Issue number3
DOIs
StatePublished - Jul 14 2000

Keywords

  • Exocytosis
  • Gene
  • Rab
  • Rab3
  • Rab3D

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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