Gold nanoshell mediated hyperthermia enhances the efficacy of radiation therapy

Parmeswaran Diagaradjane, Anil Shetty, James Wang, Andrew Elliot, Jon Schwartz, Shujun Shentu, Chul Park, Amit Deorukhkar, Jason Stafford, Sang Cho, James Tunnell, John Hazle, Sunil Krishnan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Despite convincing evidence for hyperthermic radiosensitization, the invasive means of achieving and monitoring hyperthermia and the lack of good thermal dosimetry have hindered its use in routine clinical practice. A non-invasive method to generate and monitor hyperthermia would provide renewed enthusiasm for such treatments. Near-infrared absorbing gold nanoshells have been shown to accumulate preferentially in tumors via the enhanced permeability and retention effect and have been used for thermal ablation of tumors. We evaluated the use of these nanoshells to generate hyperthermia to evaluate the anti-tumor effects of combining gold nanoshell mediated hyperthermia with radiotherapy. Laser settings were optimized for hyperthermia in a mouse xenograft model to achieve a temperature rise of 40- 41°C in the tumor periphery and 37-38°C (AT=4-5°C) deeper within the tumors. The AT measurements were verified using both thermocouple and magnetic resonance thermal imaging (MRTI) temperature measurements. Tumor re-growth delay was estimated by measuring tumor size after treatment with radiation (10Gy single dose), hyperthermia (15 minutes at 40°C), and hyperthermia followed by radiation and control. Significant difference (p <0.05) in the tumor volume doubling time was observed between the radiation group (13 days) and combination treatment group (25 days). The immunofluorescence staining for the hypoxic, proliferating cells and the vasculature corroborated our hypothesis that the radiosensitization is in part mediated by increased initial perfusion and subsequent collapse of vasculature that leads to acute inflammatory response in the tumor. The increased vascular perfusion immediately after gold nanoshell mediated hyperthermia is confirmed by dynamic contrast enhanced magnetic resonance imaging.

Original languageEnglish (US)
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications V
DOIs
StatePublished - 2008
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications V - San Jose, CA, United States
Duration: Jan 21 2008Jan 23 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6865
ISSN (Print)1605-7422

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications V
Country/TerritoryUnited States
CitySan Jose, CA
Period1/21/081/23/08

Keywords

  • Hypoxia
  • MRTI
  • Nanoshells
  • Perfusion
  • Thermoradiotherapy
  • Vascular disruption

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'Gold nanoshell mediated hyperthermia enhances the efficacy of radiation therapy'. Together they form a unique fingerprint.

Cite this