Heat shock factor 1 upregulates transcription of Epstein-Barr Virus nuclear antigen 1 by binding to a heat shock element within the BamHI-Q promoter

Feng Wei Wang, Xian Rui Wu, Wen Ju Liu, Yi Ji Liao, Sheng Lin, Yong Sheng Zong, Mu Sheng Zeng, Yi Xin Zeng, Shi Juan Mai, Dan Xie

    Research output: Contribution to journalArticle

    11 Scopus citations

    Abstract

    Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is essential for maintenance of the episome and establishment of latency. In this study, we observed that heat treatment effectively induced EBNA1 transcription in EBV-transformed B95-8 and human LCL cell lines. Although Cp is considered as the sole promoter used for the expression of EBNA1 transcripts in the lymphoblastoid cell lines, the RT-PCR results showed that the EBNA1 transcripts induced by heat treatment arise from Qp-initiated transcripts. Using bioinformatics, a high affinity and functional heat shock factor 1 (HSF1)-binding element within the -. 17/+4 oligonucleotide of the Qp was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Moreover, heat shock and exogenous HSF1 expression induced Qp activity in reporter assays. Further, RNA interference-mediated HSF1 gene silencing attenuated heat-induced EBNA1 expression in B95-8 cells. These results provide evidence that EBNA1 is a new target for the transcription factor HSF1.

    Original languageEnglish (US)
    Pages (from-to)184-191
    Number of pages8
    JournalVirology
    Volume421
    Issue number2
    DOIs
    StatePublished - Dec 20 2011

    Keywords

    • EBNA1
    • EBV
    • Heat shock factor 1
    • Q promoter

    ASJC Scopus subject areas

    • Virology

    Fingerprint Dive into the research topics of 'Heat shock factor 1 upregulates transcription of Epstein-Barr Virus nuclear antigen 1 by binding to a heat shock element within the BamHI-Q promoter'. Together they form a unique fingerprint.

  • Cite this

    Wang, F. W., Wu, X. R., Liu, W. J., Liao, Y. J., Lin, S., Zong, Y. S., Zeng, M. S., Zeng, Y. X., Mai, S. J., & Xie, D. (2011). Heat shock factor 1 upregulates transcription of Epstein-Barr Virus nuclear antigen 1 by binding to a heat shock element within the BamHI-Q promoter. Virology, 421(2), 184-191. https://doi.org/10.1016/j.virol.2011.10.001