Hepatoma-associated nonhistone chromosomal proteins are present in active chromatin

Bo Gui Wen, Anne L. Burkhard, Gilbert J. Cote, Dao Pei Huang, Jen Fu Chiu

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Recently a group of nonhistone proteins with molecular weights ranging from 180-200 K were discovered which are associated with rat hepatoma chromatin specifically (Burkhardt et al., Biochim. Biophys. Acta 781, 165-172, 1984). These hepatoma-associated nonhistone proteins appeared and increased in rats treated with a hepatocarcinogen. Two approaches were used in this study to investigate whether the hepatoma-associated nonhistone chromosomal proteins are present in actively transcribed regions. We found that the limited DNase I digestion of Morris hepatoma, 7777 chromatin released antigenic proteins not detected in normal liver chromatin digests. The association of antigenic nonhistone proteins with nuclear matrices was also studied. Using immunoblot analysis of nuclear matrices and total chromatin, the antigenic nonhistone chromosomal proteins were determined. Hepatoma-associated nonhistone protein antigens were extensively concentrated in the nuclear matrices. In the present study, the transcriptionally-active a-fetoprotein gene and the nontranscribed β-globin gene were used as gene markers to determine the transcriptionally active chromatin region. Data presented in this paper indicate that hepatoma-associated NHPs are localized in active chromatin.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume76
Issue number1
DOIs
StatePublished - Jul 1 1987

Keywords

  • active chromatin
  • hepatoma
  • nonhistone protein
  • nuclear matrix
  • α-fetoprotein gene

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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