TY - JOUR
T1 - HER2- and EGFR-specific affiprobes
T2 - Novel recombinant optical probes for cell imaging
AU - Lyakhov, Ilya
AU - Zielinski, Rafal
AU - Kuban, Monika
AU - Kramer-Marek, Gabriela
AU - Fisher, Robert
AU - Chertov, Oleg
AU - Bindu, Lakshman
AU - Capala, Jacek
PY - 2010/2/15
Y1 - 2010/2/15
N2 - The human epidermal growth factor receptors, EGFR and HER2, are members of the EGFR family of cell-surface receptors/ tyrosine kinases. EGFR- and HER2-positive cancers represent a more aggressive disease with greater likelihood of recurrence, poorer prognosis, and decreased survival rate, compared to EGFR- or HER2-negative cancers. The details of HER2 proto-oncogenic functions are not deeply understood, partially because of a restricted availability of tools for EGFR and HER2 detection (A. Sorkin and L. K. Goh, Exp. Cell Res. 2009, 315, 683-696). We have created photostable and relatively simpleto-produce imaging probes for in vitro staining of EGFR and HER2. These new reagents, called affiprobes, consist of a targeting moiety, a HER2- or EGFR-specific Affibody® molecule, and a fluorescent moiety, mCherry (red) or EGFP (green). Our flow cytometry and confocal microscopy experiments demonstrated high specificity and signal/background ratio of affiprobes. Affiprobes are able to stain both live cells and frozen tumor xenograph sections. This type of optical probe can easily be extended for targeting other cell-surface antigens/receptors.
AB - The human epidermal growth factor receptors, EGFR and HER2, are members of the EGFR family of cell-surface receptors/ tyrosine kinases. EGFR- and HER2-positive cancers represent a more aggressive disease with greater likelihood of recurrence, poorer prognosis, and decreased survival rate, compared to EGFR- or HER2-negative cancers. The details of HER2 proto-oncogenic functions are not deeply understood, partially because of a restricted availability of tools for EGFR and HER2 detection (A. Sorkin and L. K. Goh, Exp. Cell Res. 2009, 315, 683-696). We have created photostable and relatively simpleto-produce imaging probes for in vitro staining of EGFR and HER2. These new reagents, called affiprobes, consist of a targeting moiety, a HER2- or EGFR-specific Affibody® molecule, and a fluorescent moiety, mCherry (red) or EGFP (green). Our flow cytometry and confocal microscopy experiments demonstrated high specificity and signal/background ratio of affiprobes. Affiprobes are able to stain both live cells and frozen tumor xenograph sections. This type of optical probe can easily be extended for targeting other cell-surface antigens/receptors.
KW - Affiprobes
KW - Cancer
KW - EGFR
KW - HER2
KW - Imaging agents
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UR - http://www.scopus.com/inward/citedby.url?scp=76649094603&partnerID=8YFLogxK
U2 - 10.1002/cbic.200900532
DO - 10.1002/cbic.200900532
M3 - Article
C2 - 20052708
AN - SCOPUS:76649094603
SN - 1439-4227
VL - 11
SP - 345
EP - 350
JO - ChemBioChem
JF - ChemBioChem
IS - 3
ER -