Heterogeneity and diversity of IgM and IgG lupus anticoagulants in an individual with systemic lupus erythematosus

Norihiko Nakamura, Chihiro Azuma, Takashi Akamizu, Hideo Sugawa, Fumihiko Matsuda, Nobuaki Mitsuda, Tasuku Honjo, Tori Mori, Kenji Yamaji

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Abstract

From one patient with systemic lupus erythematosus retaining lupus anticoagulant (LAC), we established 6 Epstein-Barr virus-transformed human B cell clones secreting antibodies that affect the coagulation assay. Two and 4 of the clones secreted IgM and IgG antibodies, respectively. Although all 6 antibodies displayed anticardiolipin activity in ELISA, the increased binding activity in the presence of β2-glycoprotein I was limited only to the IgG antibodies. Five antibodies (two IgM and three IgG) had LAC activity which prolonged the activated partial thromboplastin time (APTT), whereas one IgG antibody shortened the APTT. Two of the IgG producing clones had an identical Ig heavy chain gene rearrangement despite their opposite effects on the coagulation assay. These results demonstrated the heterogeneity of LACs and diversity among their physiological functions.

Original languageEnglish (US)
Pages (from-to)1789-1794
Number of pages6
JournalBiochemical and biophysical research communications
Volume203
Issue number3
DOIs
StatePublished - Sep 30 1994

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Lupus Coagulation Inhibitor
Systemic Lupus Erythematosus
Immunoglobulin M
Immunoglobulin G
Antibodies
Partial Thromboplastin Time
Clone Cells
Thromboplastin
Coagulation
Assays
Clone cells
Anticardiolipin Antibodies
Immunoglobulin Heavy Chains
Gene Rearrangement
Human Herpesvirus 4
Viruses
Glycoproteins
B-Lymphocytes
Genes
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Heterogeneity and diversity of IgM and IgG lupus anticoagulants in an individual with systemic lupus erythematosus. / Nakamura, Norihiko; Azuma, Chihiro; Akamizu, Takashi; Sugawa, Hideo; Matsuda, Fumihiko; Mitsuda, Nobuaki; Honjo, Tasuku; Mori, Tori; Yamaji, Kenji.

In: Biochemical and biophysical research communications, Vol. 203, No. 3, 30.09.1994, p. 1789-1794.

Research output: Contribution to journalArticle

Nakamura, Norihiko ; Azuma, Chihiro ; Akamizu, Takashi ; Sugawa, Hideo ; Matsuda, Fumihiko ; Mitsuda, Nobuaki ; Honjo, Tasuku ; Mori, Tori ; Yamaji, Kenji. / Heterogeneity and diversity of IgM and IgG lupus anticoagulants in an individual with systemic lupus erythematosus. In: Biochemical and biophysical research communications. 1994 ; Vol. 203, No. 3. pp. 1789-1794.
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