Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro

Tian Liao, Yuan Jin Wang, Jia Qian Hu, Yu Wang, Li Tao Han, Ben Ma, Rong Liang Shi, Ning Qu, Wen Jun Wei, Qing Guan, Jun Xiang, Jia Ying Chen, Guo Hua Sun, Duan Shu Li, Xiang Ming Mu, Qing Hai Ji

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

KMT5A (known as PR-Set7/9, SETD8 and SET8), a member of the SET domain containing methyltransferase family specifically targeting H4K20 for methylation, has been implicated in multiple biological processes. In the present study, we identified that KMT5A was elevated in 50 pairs of papillary thyroid cancer tissue samples and in cell lines K1 and TPC-1 by qRT-PCR and western blotting, as well as by immunohistochemical staining. CCK-8 assay and flow cytometric analysis revealed that inhibition of KMT5A attenuated proliferation and induced apoptosis. Transwell assays revealed that cell migration and invasion were suppressed in KMT5A-knockdown cells. Moreover, the inhibition of KMT5A arrested the cell cycle in the G1/S phase of papillary thyroid cancer cells. The TCGA data revealed that elevated KMT5A expression was significantly correlated with extrathyroidal extension, lymph node metastasis and advanced pathological stage of papillary thyroid cancer. Furthermore, we observed that inhibition of KMT5A suppressed the expression of SREBP1, SCD, FASN and ACC, key molecules involved in lipid metabolism and decreased the level of malondialdehyde in papillary thyroid cancer cells. In conclusion, KMT5A may be a novel oncogenic factor, specifically a regulator for lipid metabolism in papillary thyroid carcinoma.

Original languageEnglish (US)
Pages (from-to)2185-2192
Number of pages8
JournalOncology reports
Volume39
Issue number5
DOIs
StatePublished - May 2018

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Lipid Metabolism
Carcinogenesis
Genes
Cell Migration Assays
Biological Phenomena
Sincalide
Methyltransferases
G1 Phase
Malondialdehyde
S Phase
Methylation
Cell Cycle
Lymph Nodes
Western Blotting
Papillary Thyroid cancer
histone methyltransferase
In Vitro Techniques
Apoptosis
Staining and Labeling
Neoplasm Metastasis

Keywords

  • KMT5A
  • Lipid metabolism
  • Papillary thyroid cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro. / Liao, Tian; Wang, Yuan Jin; Hu, Jia Qian; Wang, Yu; Han, Li Tao; Ma, Ben; Shi, Rong Liang; Qu, Ning; Wei, Wen Jun; Guan, Qing; Xiang, Jun; Chen, Jia Ying; Sun, Guo Hua; Li, Duan Shu; Mu, Xiang Ming; Ji, Qing Hai.

In: Oncology reports, Vol. 39, No. 5, 05.2018, p. 2185-2192.

Research output: Contribution to journalArticle

Liao, T, Wang, YJ, Hu, JQ, Wang, Y, Han, LT, Ma, B, Shi, RL, Qu, N, Wei, WJ, Guan, Q, Xiang, J, Chen, JY, Sun, GH, Li, DS, Mu, XM & Ji, QH 2018, 'Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro', Oncology reports, vol. 39, no. 5, pp. 2185-2192. https://doi.org/10.3892/or.2018.6295
Liao, Tian ; Wang, Yuan Jin ; Hu, Jia Qian ; Wang, Yu ; Han, Li Tao ; Ma, Ben ; Shi, Rong Liang ; Qu, Ning ; Wei, Wen Jun ; Guan, Qing ; Xiang, Jun ; Chen, Jia Ying ; Sun, Guo Hua ; Li, Duan Shu ; Mu, Xiang Ming ; Ji, Qing Hai. / Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro. In: Oncology reports. 2018 ; Vol. 39, No. 5. pp. 2185-2192.
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