Hydrogen peroxide induces LFA-1-dependent neutrophil adherence to cardiac myocytes

Huifang Lu, Keith Youker, Christie Ballantyne, Mark Entman, C. Wayne Smith

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Adult cardiac myocytes express intercellular adhesion molecule (ICAM)-1 in response to cytokine stimulation. This allows stable adhesion of chemotactically stimulated but not unstimulated neutrophils. In the current study, we demonstrated that brief exposure of ICAM-1-expressing cardiac myocytes to H2O2 promoted transient adhesive interactions between myocytes and neutrophils without added chemotactic factors. This transient adhesion differed in two ways from the stable adhesion promoted by exogenous chemotactic factors. It occurred more rapidly, peaking within 15 min, and it was dependent on leukocyte function-associated antigen (LFA)-1 (CD11a/CD18) on the neutrophil interacting with ICAM-1 on the myocyte. In contrast, chemotactic factor-induced adhesion peaked at 60 min and was dependent on Mac-1 (CD11b/CD18). The transient adhesion could be completely inhibited by platelet-activating factor (PAF)-receptor antagonists WEB-2086 and SDZ-64- 412. These results indicate that canine neutrophils may utilize both LFA-1 and Mac-1 to adhere to adult cardiac myocytes, with LFA-1 triggered by a PAF- like activity induced in myocytes by H2O2.

Original languageEnglish (US)
Pages (from-to)H835-H842
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume278
Issue number3 47-3
StatePublished - Mar 1 2000

Fingerprint

Lymphocyte Function-Associated Antigen-1
Cardiac Myocytes
Hydrogen Peroxide
Chemotactic Factors
Neutrophils
Intercellular Adhesion Molecule-1
Muscle Cells
WEB 2086
Platelet Activating Factor
Adhesives
Canidae
Cytokines

Keywords

  • Inflammation
  • Reactive oxygen

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Hydrogen peroxide induces LFA-1-dependent neutrophil adherence to cardiac myocytes. / Lu, Huifang; Youker, Keith; Ballantyne, Christie; Entman, Mark; Smith, C. Wayne.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 278, No. 3 47-3, 01.03.2000, p. H835-H842.

Research output: Contribution to journalArticle

Lu, Huifang ; Youker, Keith ; Ballantyne, Christie ; Entman, Mark ; Smith, C. Wayne. / Hydrogen peroxide induces LFA-1-dependent neutrophil adherence to cardiac myocytes. In: American Journal of Physiology - Heart and Circulatory Physiology. 2000 ; Vol. 278, No. 3 47-3. pp. H835-H842.
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