Hypochlorite-modified LDL: Chemotactic potential and chemokine induction in human monocytes

Christian Woenckhaus, Andreas Kaufmann, Delia Bußfeld, Diethard Gemsa, Hans Sprenger, Hermann-Josef Gröne

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Within blood vessels the accumulation of monocytes/macrophages at sites of modified lipoproteins is an important feature in atherosclerosis. Recently the presence of LDL and other proteins modified by hypochlorous acid (HOCI- LDL) was demonstrated in human atherosclerotic vessels and human inflammatory kidney disease by immunhistology and protein chemistry. Chemokines contribute to a specific and directed migration of inflammatory cells. IL-8 (α- chemokine) attracts mainly neutrophils and distinct T-cell subsets while MCP- 1 (β-chemokine) preferentially acts on monocytes/macrophages. In the present study it was postulated that HOCI-LDL may induce and amplify inflammatory reactions by the induction of chemokine synthesis in local monocytes. After exposure of human monocytes to HOCI-LDL, it was found that mRNA and protein of the chemokine IL-8 was strongly induced, while the chemokine MCP-1 was not. HOCI-LDL itself led to a chemotactic migration of neutrophils. A chemotactic response of human monocytes toward HOCI-LDL was not detectable. We propose that HOCI-LDL may represent a form of LDL modification in the atherosclerotic process which initiates leukocyte infiltration; these mononuclear cells have been observed in the early stages of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalClinical Immunology and Immunopathology
Volume86
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

Hypochlorous Acid
Chemokines
Monocytes
Interleukin-8
Atherosclerosis
Neutrophils
Macrophages
Proteins
Kidney Diseases
T-Lymphocyte Subsets
oxidized low density lipoprotein
Lipoproteins
Cell Movement
Blood Vessels
Leukocytes
Messenger RNA

Keywords

  • Atherosclerosis
  • Chemokines
  • Chemotaxis
  • Hypochlorite-modified LDL
  • IL-8
  • Low-density lipoprotein
  • MCP-1
  • Monocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

Cite this

Hypochlorite-modified LDL : Chemotactic potential and chemokine induction in human monocytes. / Woenckhaus, Christian; Kaufmann, Andreas; Bußfeld, Delia; Gemsa, Diethard; Sprenger, Hans; Gröne, Hermann-Josef.

In: Clinical Immunology and Immunopathology, Vol. 86, No. 1, 01.01.1998, p. 27-33.

Research output: Contribution to journalArticle

Woenckhaus, Christian ; Kaufmann, Andreas ; Bußfeld, Delia ; Gemsa, Diethard ; Sprenger, Hans ; Gröne, Hermann-Josef. / Hypochlorite-modified LDL : Chemotactic potential and chemokine induction in human monocytes. In: Clinical Immunology and Immunopathology. 1998 ; Vol. 86, No. 1. pp. 27-33.
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