Identification of three novel noncoding RNAs from Drosophila melanogaster

Hualiang He, Hui Zhou, Zhendong Xiao, Xianfen Zeng, Junyu Chen, Tao Zheng, Lianghu Qu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Three novel small noncoding RNAs were identified from the conserved intronic regions of D. melanogaster by using comparative genomics method and molecular biology approach. One is a novel snoRNA in a combinated way, which displays structural features typical of C/D box snoRNA family and possesses a 10-nt-long rRNA antisense element for guiding the 2′-O-methylation of the D. melanogaster 28S rRNA at C2673. The other two are miRNAs whose predicted precursors adopt a stemloop structure characteristic of known miRNA. The two miRNAs genes appear to have ubiquitous expression profiles with ∼23-nt RNA transcripts detected by Northern blotting. Our study revealed 396 multi-species intronic conserved sequences (MCIS) that nested in the introns with a length from 100 to 500 bp. In addition to small RNA coding, the MCIS might function as cis-acting elements involved in gene transcription or post-transcriptional processing.

Original languageEnglish (US)
Pages (from-to)2737-2742
Number of pages6
JournalChinese Science Bulletin
Volume51
Issue number22
DOIs
StatePublished - Nov 1 2006

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Keywords

  • Box C/D snoRNA
  • Drosophila melanogaster
  • Intron
  • MCIS
  • MiRNA

ASJC Scopus subject areas

  • General

Cite this

He, H., Zhou, H., Xiao, Z., Zeng, X., Chen, J., Zheng, T., & Qu, L. (2006). Identification of three novel noncoding RNAs from Drosophila melanogaster. Chinese Science Bulletin, 51(22), 2737-2742. https://doi.org/10.1007/s11434-006-2202-1