IgE Binding Patterns to German Cockroach Whole Body Extract in Korean Atopic Asthmatic Children

Soo Young Lee, Dong Soo Kim, Kyu Earn Kim, Byung Joo Jeaung, Ki Young Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It is widely known that the cockroach is an inhalant allergen in atopic asthma and allergic rhinitis. Even though Bla g I and Bla g II are considered as the major allergens, several relatively high-molecular weight (MW) cockroach allergens have also been recently identified by IgE-immunoblot in western countries. However, the environmental control and diagnostic tests mainly focussed on Bla g I and Bla g II. Furthermore there is no data about major IgE-binding cockroach antigens in Korea. We performed this study to identify the major German cockroach allergens in Korean atopic children. By the results of allergy skin tests, 14 children with atopic asthma (9 were cockroach-sensitive and 5 were cockroach-nonsensitive atopics) were enrolled in this study. We conducted IgE immunoblot and autoradiographic analysis using Yonsei-extract of German cockroach antigen produced in our laboratory, individual sera from 9 cockroach-sensitive children, and the pooled sera of 5 house-dust-mites-only-sensitive children. We performed an allergic skin test to cockroach mix, and a radioallergosorbent test (RAST) using German cockroach crude extract on all subjects. German cockroach-specific IgE was detected in 6 out of 9 subjects by RAST. We identified at least 15 IgE-binding protein bands, and among them, the components of MWs of 76, 64, 50, 38, and <14 kilodaltons (kDa) were the major German cockroach allergens in study subjects. Therefore, Bla g I (25 ∼30 kDa) and Bla g II (36 kDa) could not be the absolute indicators of German cockroach sensitization and parameters of environmental control.

Original languageEnglish (US)
Pages (from-to)409-416
Number of pages8
JournalYonsei Medical Journal
Volume39
Issue number5
DOIs
StatePublished - Jan 1 1998

Fingerprint

Blattellidae
Cockroaches
Immunoglobulin E
Allergens
Radioallergosorbent Test
Skin Tests
Asthma
Galectin 3
Antigens
Pyroglyphidae
Korea
Complex Mixtures
Serum
Routine Diagnostic Tests
Hypersensitivity
Molecular Weight
Western Blotting

Keywords

  • Atopic asthmatic children
  • German cockroach extract
  • IgE binding patterns

ASJC Scopus subject areas

  • Medicine(all)

Cite this

IgE Binding Patterns to German Cockroach Whole Body Extract in Korean Atopic Asthmatic Children. / Lee, Soo Young; Kim, Dong Soo; Kim, Kyu Earn; Jeaung, Byung Joo; Lee, Ki Young.

In: Yonsei Medical Journal, Vol. 39, No. 5, 01.01.1998, p. 409-416.

Research output: Contribution to journalArticle

Lee, Soo Young ; Kim, Dong Soo ; Kim, Kyu Earn ; Jeaung, Byung Joo ; Lee, Ki Young. / IgE Binding Patterns to German Cockroach Whole Body Extract in Korean Atopic Asthmatic Children. In: Yonsei Medical Journal. 1998 ; Vol. 39, No. 5. pp. 409-416.
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