@inbook{97dc77d8912945b6a28a1ff80d760e65,
title = "Immunoguided Microdissection Techniques",
abstract = "Over the past 15 years, laser-based microdissection has improved the precision by which scientists can procure cells of interest from a heterogeneous tissue section. However, for studies that require a large amount of material (e.g., proteomics) or for cells that are scattered and difficult to identify by standard histological stains, an immunostain-based, automated approach becomes essential. In this chapter, we discuss the use of immunohistochemistry (IHC) and immunofluorescence (IF) to guide the microdissection process via manual and software-driven auto-dissection methods. Although technical challenges still exist with these innovative approaches, we present here methods and protocols to successfully perform immuno-based microdissection on commercially available laser dissection systems.",
keywords = "Automation, Immunohistochemistry, Microdissection",
author = "Tangrea, {Michael A.} and Hanson, {Jeffrey C.} and Bonner, {Robert F.} and Pohida, {Thomas J.} and Jaime Rodriguez-Canales and Emmert-Buck, {Michael R.}",
note = "Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",
year = "2011",
doi = "10.1007/978-1-61779-163-5_4",
language = "English (US)",
isbn = "9781617791628",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "57--66",
booktitle = "Laser Capture Microdissection",
}