Impact of obesity on airway and lung parenchyma remodeling in experimental chronic allergic asthma

Simone A. Saraiva; Adriana L. Silva; Débora G. Xisto; Soraia C. Abreu; Johnatas D. Silva; Pedro L. Silva; Tatiana P.F. Teixeira; Edwin R. Parra; Ana Laura N. Carvalho; Raquel Annoni; Thais Mauad; Vera L. Capelozzi; Patricia M.R. Silva; Marco A. Martins; Patricia R.M. Rocco

doi:10.1016/j.resp.2011.03.019

Impact of obesity on airway and lung parenchyma remodeling in experimental chronic allergic asthma

Simone A. Saraiva, Adriana L. Silva, Débora G. Xisto, Soraia C. Abreu, Johnatas D. Silva, Pedro L. Silva, Tatiana P.F. Teixeira, Edwin R. Parra, Ana Laura N. Carvalho, Raquel Annoni, Thais Mauad, Vera L. Capelozzi, Patricia M.R. Silva, Marco A. Martins, Patricia R.M. Rocco

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The impact of obesity on the inflammatory process has been described in asthma, however little is known about the influence of diet-induced obesity on lung remodeling. For this purpose, 56 recently weaned A/J mice were randomly divided into 2 groups. In the C group, mice were fed a standard chow diet, while OB animals received isocaloric high-fat diet to reach 1.5 of the mean body weight of C. After 12 weeks, each group was further randomized to be sensitized and challenged with ovalbumin (OVA) or saline. Twenty-four hours after the last challenge, collagen fiber content in airways and lung parenchyma, the volume proportion of smooth muscle-specific actin in alveolar ducts and terminal bronchiole, and the number of eosinophils in bronchoalveolar lavage fluid were higher in OB-OVA than C-OVA. In conclusion, diet-induced obesity enhanced lung remodeling resulting in higher airway responsiveness in the present experimental chronic allergic asthma.

Original language	English (US)
Pages (from-to)	141-148
Number of pages	8
Journal	Respiratory Physiology and Neurobiology
Volume	177
Issue number	2
DOIs	https://doi.org/10.1016/j.resp.2011.03.019
State	Published - Jul 31 2011
Externally published	Yes

Keywords

Airway
Asthma
Collagen fiber
Electron microscopy
Eosinophil

ASJC Scopus subject areas

General Neuroscience
Physiology
Pulmonary and Respiratory Medicine

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10.1016/j.resp.2011.03.019

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Saraiva, S. A., Silva, A. L., Xisto, D. G., Abreu, S. C., Silva, J. D., Silva, P. L., Teixeira, T. P. F., Parra, E. R., Carvalho, A. L. N., Annoni, R., Mauad, T., Capelozzi, V. L., Silva, P. M. R., Martins, M. A., & Rocco, P. R. M. (2011). Impact of obesity on airway and lung parenchyma remodeling in experimental chronic allergic asthma. Respiratory Physiology and Neurobiology, 177(2), 141-148. https://doi.org/10.1016/j.resp.2011.03.019

Saraiva, SA, Silva, AL, Xisto, DG, Abreu, SC, Silva, JD, Silva, PL, Teixeira, TPF, Parra, ER, Carvalho, ALN, Annoni, R, Mauad, T, Capelozzi, VL, Silva, PMR, Martins, MA & Rocco, PRM 2011, 'Impact of obesity on airway and lung parenchyma remodeling in experimental chronic allergic asthma', Respiratory Physiology and Neurobiology, vol. 177, no. 2, pp. 141-148. https://doi.org/10.1016/j.resp.2011.03.019

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title = "Impact of obesity on airway and lung parenchyma remodeling in experimental chronic allergic asthma",

abstract = "The impact of obesity on the inflammatory process has been described in asthma, however little is known about the influence of diet-induced obesity on lung remodeling. For this purpose, 56 recently weaned A/J mice were randomly divided into 2 groups. In the C group, mice were fed a standard chow diet, while OB animals received isocaloric high-fat diet to reach 1.5 of the mean body weight of C. After 12 weeks, each group was further randomized to be sensitized and challenged with ovalbumin (OVA) or saline. Twenty-four hours after the last challenge, collagen fiber content in airways and lung parenchyma, the volume proportion of smooth muscle-specific actin in alveolar ducts and terminal bronchiole, and the number of eosinophils in bronchoalveolar lavage fluid were higher in OB-OVA than C-OVA. In conclusion, diet-induced obesity enhanced lung remodeling resulting in higher airway responsiveness in the present experimental chronic allergic asthma.",

keywords = "Airway, Asthma, Collagen fiber, Electron microscopy, Eosinophil",

author = "Saraiva, {Simone A.} and Silva, {Adriana L.} and Xisto, {D{\'e}bora G.} and Abreu, {Soraia C.} and Silva, {Johnatas D.} and Silva, {Pedro L.} and Teixeira, {Tatiana P.F.} and Parra, {Edwin R.} and Carvalho, {Ana Laura N.} and Raquel Annoni and Thais Mauad and Capelozzi, {Vera L.} and Silva, {Patricia M.R.} and Martins, {Marco A.} and Rocco, {Patricia R.M.}",

note = "Funding Information: This study was supported by Centers of Excellence Program (PRONEXFAPERJ) , Brazilian Council for Scientific and Technological Development (CNPq) , Rio de Janeiro State Research Supporting Foundation (FAPERJ) , Coordination for the Improvement of Higher Education Personnel (CAPES) , and S{\~a}o Paulo State Research Supporting Foundation (FAPESP) .",

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doi = "10.1016/j.resp.2011.03.019",

language = "English (US)",

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T1 - Impact of obesity on airway and lung parenchyma remodeling in experimental chronic allergic asthma

AU - Saraiva, Simone A.

AU - Silva, Adriana L.

AU - Xisto, Débora G.

AU - Abreu, Soraia C.

AU - Silva, Johnatas D.

AU - Silva, Pedro L.

AU - Teixeira, Tatiana P.F.

AU - Parra, Edwin R.

AU - Carvalho, Ana Laura N.

AU - Annoni, Raquel

AU - Mauad, Thais

AU - Capelozzi, Vera L.

AU - Silva, Patricia M.R.

AU - Martins, Marco A.

AU - Rocco, Patricia R.M.

N1 - Funding Information: This study was supported by Centers of Excellence Program (PRONEXFAPERJ) , Brazilian Council for Scientific and Technological Development (CNPq) , Rio de Janeiro State Research Supporting Foundation (FAPERJ) , Coordination for the Improvement of Higher Education Personnel (CAPES) , and São Paulo State Research Supporting Foundation (FAPESP) .

PY - 2011/7/31

Y1 - 2011/7/31

N2 - The impact of obesity on the inflammatory process has been described in asthma, however little is known about the influence of diet-induced obesity on lung remodeling. For this purpose, 56 recently weaned A/J mice were randomly divided into 2 groups. In the C group, mice were fed a standard chow diet, while OB animals received isocaloric high-fat diet to reach 1.5 of the mean body weight of C. After 12 weeks, each group was further randomized to be sensitized and challenged with ovalbumin (OVA) or saline. Twenty-four hours after the last challenge, collagen fiber content in airways and lung parenchyma, the volume proportion of smooth muscle-specific actin in alveolar ducts and terminal bronchiole, and the number of eosinophils in bronchoalveolar lavage fluid were higher in OB-OVA than C-OVA. In conclusion, diet-induced obesity enhanced lung remodeling resulting in higher airway responsiveness in the present experimental chronic allergic asthma.

AB - The impact of obesity on the inflammatory process has been described in asthma, however little is known about the influence of diet-induced obesity on lung remodeling. For this purpose, 56 recently weaned A/J mice were randomly divided into 2 groups. In the C group, mice were fed a standard chow diet, while OB animals received isocaloric high-fat diet to reach 1.5 of the mean body weight of C. After 12 weeks, each group was further randomized to be sensitized and challenged with ovalbumin (OVA) or saline. Twenty-four hours after the last challenge, collagen fiber content in airways and lung parenchyma, the volume proportion of smooth muscle-specific actin in alveolar ducts and terminal bronchiole, and the number of eosinophils in bronchoalveolar lavage fluid were higher in OB-OVA than C-OVA. In conclusion, diet-induced obesity enhanced lung remodeling resulting in higher airway responsiveness in the present experimental chronic allergic asthma.

KW - Airway

KW - Asthma

KW - Collagen fiber

KW - Electron microscopy

KW - Eosinophil

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Impact of obesity on airway and lung parenchyma remodeling in experimental chronic allergic asthma

Abstract

Keywords

ASJC Scopus subject areas

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