Improved immune response by ID-pVAC: A secretory DNA vaccine construct delivered by PLG micro particles against foot and mouth disease in guinea pigs

Sameer K. Jadav; K. Siva Reddy; B. R. Rashmi; H. J. Dechamma; K. Ganesh; V. V.S. Suryanarayana; G. R. Reddy

doi:10.1016/j.rvsc.2010.08.011

Improved immune response by ID-pVAC: A secretory DNA vaccine construct delivered by PLG micro particles against foot and mouth disease in guinea pigs

Sameer K. Jadav, K. Siva Reddy, B. R. Rashmi, H. J. Dechamma, K. Ganesh, V. V.S. Suryanarayana, G. R. Reddy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Foot and mouth disease (FMD) outbreaks usually have devastating effects on the economy of countries were disease is endemic due to direct and indirect cost; most of them related to international trade embargoes of animals and animal products. Although currently used inactivated vaccine provides protection, it has several drawbacks like short duration of immunity, and the requirement for containment facilities. A DNA vaccine construct which expresses the secretary antigens, delivered through micro particles could be one of the alternate approaches to overcome these limitations. Present study is envisaged to prepare a DNA vaccine construct containing the VP1 sequence of FMDV serotype O in pVAC vector. DNA vaccine was formulated by adsorbing plasmid DNA construct on cationic micro particles and administered in guinea pigs @25μg DNA vaccine construct per animal intramuscularly. Sera samples collected were analyzed by sandwich ELISA and SNT, shown enhanced immune response in PLG adjuvanted DNA vaccine. MTT and 3H Thymidine incorporation have shown good CMI responses to PLG adjuvanted DNA. When challenged with 100 gpid50 of homologous virus 5 of the six animals were protected.

Original language	English (US)
Pages (from-to)	86-89
Number of pages	4
Journal	Research in Veterinary Science
Volume	91
Issue number	1
DOIs	https://doi.org/10.1016/j.rvsc.2010.08.011
State	Published - Aug 2011
Externally published	Yes

Keywords

DNA vaccine

ASJC Scopus subject areas

General Veterinary

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10.1016/j.rvsc.2010.08.011

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title = "Improved immune response by ID-pVAC: A secretory DNA vaccine construct delivered by PLG micro particles against foot and mouth disease in guinea pigs",

abstract = "Foot and mouth disease (FMD) outbreaks usually have devastating effects on the economy of countries were disease is endemic due to direct and indirect cost; most of them related to international trade embargoes of animals and animal products. Although currently used inactivated vaccine provides protection, it has several drawbacks like short duration of immunity, and the requirement for containment facilities. A DNA vaccine construct which expresses the secretary antigens, delivered through micro particles could be one of the alternate approaches to overcome these limitations. Present study is envisaged to prepare a DNA vaccine construct containing the VP1 sequence of FMDV serotype O in pVAC vector. DNA vaccine was formulated by adsorbing plasmid DNA construct on cationic micro particles and administered in guinea pigs @25μg DNA vaccine construct per animal intramuscularly. Sera samples collected were analyzed by sandwich ELISA and SNT, shown enhanced immune response in PLG adjuvanted DNA vaccine. MTT and 3H Thymidine incorporation have shown good CMI responses to PLG adjuvanted DNA. When challenged with 100 gpid50 of homologous virus 5 of the six animals were protected.",

keywords = "DNA vaccine",

author = "Jadav, {Sameer K.} and Reddy, {K. Siva} and Rashmi, {B. R.} and Dechamma, {H. J.} and K. Ganesh and Suryanarayana, {V. V.S.} and Reddy, {G. R.}",

note = "Funding Information: The authors thanks the Director, IVRI, Izatnagar and Joint Director, IVRI, Bangalore for providing all the facilities to carry out this work and DBT Govt of India for financial assistance.",

year = "2011",

month = aug,

doi = "10.1016/j.rvsc.2010.08.011",

language = "English (US)",

volume = "91",

pages = "86--89",

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T2 - A secretory DNA vaccine construct delivered by PLG micro particles against foot and mouth disease in guinea pigs

AU - Jadav, Sameer K.

AU - Reddy, K. Siva

AU - Rashmi, B. R.

AU - Dechamma, H. J.

AU - Ganesh, K.

AU - Suryanarayana, V. V.S.

AU - Reddy, G. R.

N1 - Funding Information: The authors thanks the Director, IVRI, Izatnagar and Joint Director, IVRI, Bangalore for providing all the facilities to carry out this work and DBT Govt of India for financial assistance.

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N2 - Foot and mouth disease (FMD) outbreaks usually have devastating effects on the economy of countries were disease is endemic due to direct and indirect cost; most of them related to international trade embargoes of animals and animal products. Although currently used inactivated vaccine provides protection, it has several drawbacks like short duration of immunity, and the requirement for containment facilities. A DNA vaccine construct which expresses the secretary antigens, delivered through micro particles could be one of the alternate approaches to overcome these limitations. Present study is envisaged to prepare a DNA vaccine construct containing the VP1 sequence of FMDV serotype O in pVAC vector. DNA vaccine was formulated by adsorbing plasmid DNA construct on cationic micro particles and administered in guinea pigs @25μg DNA vaccine construct per animal intramuscularly. Sera samples collected were analyzed by sandwich ELISA and SNT, shown enhanced immune response in PLG adjuvanted DNA vaccine. MTT and 3H Thymidine incorporation have shown good CMI responses to PLG adjuvanted DNA. When challenged with 100 gpid50 of homologous virus 5 of the six animals were protected.

AB - Foot and mouth disease (FMD) outbreaks usually have devastating effects on the economy of countries were disease is endemic due to direct and indirect cost; most of them related to international trade embargoes of animals and animal products. Although currently used inactivated vaccine provides protection, it has several drawbacks like short duration of immunity, and the requirement for containment facilities. A DNA vaccine construct which expresses the secretary antigens, delivered through micro particles could be one of the alternate approaches to overcome these limitations. Present study is envisaged to prepare a DNA vaccine construct containing the VP1 sequence of FMDV serotype O in pVAC vector. DNA vaccine was formulated by adsorbing plasmid DNA construct on cationic micro particles and administered in guinea pigs @25μg DNA vaccine construct per animal intramuscularly. Sera samples collected were analyzed by sandwich ELISA and SNT, shown enhanced immune response in PLG adjuvanted DNA vaccine. MTT and 3H Thymidine incorporation have shown good CMI responses to PLG adjuvanted DNA. When challenged with 100 gpid50 of homologous virus 5 of the six animals were protected.

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Improved immune response by ID-pVAC: A secretory DNA vaccine construct delivered by PLG micro particles against foot and mouth disease in guinea pigs

Abstract

Keywords

ASJC Scopus subject areas

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