In Vitro Assay to Study CLL and Monocyte Interactions

Maria Teresa S Bertilaccio, Ronghua Zhang, Priyanka Banerjee, Varsha V Gandhi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Investigations focusing on CLL and microenvironment interaction allow understanding role of each component of the microenvironment. In vitro cell depletion assay we described here enables us to evaluate the depletion of CLL cells and monocyte populations upon treatment with drugs targeting the interactions between CLL cells and monocytes. The assay is based on a quantitative multi-color flow cytometry analysis and, when combined to fluorescence-activated cell sorting and RT-PCR, it allows the isolation of CLL/monocyte cells and the further characterization of apoptotic and/or inflammatory pathways induced eventually on CLL cells and on monocytes.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages113-119
Number of pages7
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1881
ISSN (Print)1064-3745

Fingerprint

Monocytes
Flow Cytometry
Drug Delivery Systems
Drug Interactions
Color
In Vitro Techniques
Polymerase Chain Reaction
Population
Therapeutics

Keywords

  • Antibodies
  • Flow cytometry
  • Fluorescence-activated cell sorting
  • RNA
  • RT-PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bertilaccio, M. T. S., Zhang, R., Banerjee, P., & Gandhi, V. V. (2019). In Vitro Assay to Study CLL and Monocyte Interactions. In Methods in Molecular Biology (pp. 113-119). (Methods in Molecular Biology; Vol. 1881). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8876-1_9

In Vitro Assay to Study CLL and Monocyte Interactions. / Bertilaccio, Maria Teresa S; Zhang, Ronghua; Banerjee, Priyanka; Gandhi, Varsha V.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 113-119 (Methods in Molecular Biology; Vol. 1881).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bertilaccio, MTS, Zhang, R, Banerjee, P & Gandhi, VV 2019, In Vitro Assay to Study CLL and Monocyte Interactions. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1881, Humana Press Inc., pp. 113-119. https://doi.org/10.1007/978-1-4939-8876-1_9
Bertilaccio MTS, Zhang R, Banerjee P, Gandhi VV. In Vitro Assay to Study CLL and Monocyte Interactions. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 113-119. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8876-1_9
Bertilaccio, Maria Teresa S ; Zhang, Ronghua ; Banerjee, Priyanka ; Gandhi, Varsha V. / In Vitro Assay to Study CLL and Monocyte Interactions. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 113-119 (Methods in Molecular Biology).
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