In Vitro Assay to Study CLL and Monocyte Interactions

Maria Teresa Sabrina Bertilaccio, Ronghua Zhang, Priyanka Banerjee, Varsha Gandhi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Investigations focusing on CLL and microenvironment interaction allow understanding role of each component of the microenvironment. In vitro cell depletion assay we described here enables us to evaluate the depletion of CLL cells and monocyte populations upon treatment with drugs targeting the interactions between CLL cells and monocytes. The assay is based on a quantitative multi-color flow cytometry analysis and, when combined to fluorescence-activated cell sorting and RT-PCR, it allows the isolation of CLL/monocyte cells and the further characterization of apoptotic and/or inflammatory pathways induced eventually on CLL cells and on monocytes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages113-119
Number of pages7
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1881
ISSN (Print)1064-3745

Keywords

  • Antibodies
  • Flow cytometry
  • Fluorescence-activated cell sorting
  • RNA
  • RT-PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'In Vitro Assay to Study CLL and Monocyte Interactions'. Together they form a unique fingerprint.

Cite this