In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay

Nick A. Kuburich, Petra den Hollander, Abhijeet P. Deshmukh, Suhas Vasaikar, Robiya Joseph, Max S. Wicha, Sendurai A. Mani

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. In this chapter, we provide a detailed method for the quantification of CSCs in vitro through mammosphere formation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages509-513
Number of pages5
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2429
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cancer stem cells (CSC)
  • Mammosphere
  • Stemness
  • Suspension
  • Tumorsphere

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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