In vitro selection of mRNA display libraries containing an unnatural amino acid

Shuwei Li, Steven Millward, Richard Roberts

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

The incorporation of unnatural amino acid into selectable, amplifiable peptide and protein libraries expands the chemical diversity of such libraries, thus considerably facilitating the process of obtaining ligands with improved properties (affinity, specificity, and function), particularly against therapeutically interesting targets. Here, we report that biocytin, a biotin derivative of lysine, can be inserted into an mRNA-protein fusion molecule through amber stop codon suppression. We also demonstrate that templates containing the codon corresponding to the biocytin tRNA (a UAG stop codon) can be enriched by iterative cycles of selection against a streptavidin agarose matrix.

Original languageEnglish (US)
Pages (from-to)9972-9973
Number of pages2
JournalJournal of the American Chemical Society
Volume124
Issue number34
DOIs
StatePublished - Aug 28 2002
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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