Abstract
A hyperspectral imaging system using a liquid-crystal tunable filter (LCTF) was constructed for the purpose of in vivo optical imaging of oral neoplasia. The system operates in fluorescence mode and has the dual capability of capturing high quality widefield images and detecting fluorescence emission spectra from arbitrary locations within the captured field of view (FOV). The system was calibrated and evaluated for spectral resolution and accuracy. In vivo hyperspectral images were obtained from two normal volunteers and two patients with confirmed oral malignancy. Normal volunteer measurements revealed differences in intensity and lineshape of spectra between different anatomic locations, but intensity and lineshape were similar between different measurement sites from the same anatomic location. Measurements from normal and neoplastic areas of two patients with previously confirmed oral neoplasia showed differences in intensity, lineshape, and location of peak intensity. We have demonstrated that this system can provide both high quality widefield images, and spectral information at chosen locations within the field of view.
Original language | English (US) |
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Article number | 71690J |
Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 7169 |
DOIs | |
State | Published - 2009 |
Event | Advanced Biomedical and Clinical Diagnostic Systems VII - San Jose, CA, United States Duration: Jan 25 2009 → Jan 26 2009 |
Keywords
- Cancer Detection
- Fluorescence
- Hyperspectral
- Imaging
- Oral Cancer
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging