In vivo fluorescence hyperspectral imaging of oral neoplasia

Darren Roblyer, Cristina Kurachi, Ann M. Gillenwater, Rebecca Richards-Kortum

Research output: Contribution to journalConference articlepeer-review

22 Scopus citations

Abstract

A hyperspectral imaging system using a liquid-crystal tunable filter (LCTF) was constructed for the purpose of in vivo optical imaging of oral neoplasia. The system operates in fluorescence mode and has the dual capability of capturing high quality widefield images and detecting fluorescence emission spectra from arbitrary locations within the captured field of view (FOV). The system was calibrated and evaluated for spectral resolution and accuracy. In vivo hyperspectral images were obtained from two normal volunteers and two patients with confirmed oral malignancy. Normal volunteer measurements revealed differences in intensity and lineshape of spectra between different anatomic locations, but intensity and lineshape were similar between different measurement sites from the same anatomic location. Measurements from normal and neoplastic areas of two patients with previously confirmed oral neoplasia showed differences in intensity, lineshape, and location of peak intensity. We have demonstrated that this system can provide both high quality widefield images, and spectral information at chosen locations within the field of view.

Original languageEnglish (US)
Article number71690J
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7169
DOIs
StatePublished - 2009
EventAdvanced Biomedical and Clinical Diagnostic Systems VII - San Jose, CA, United States
Duration: Jan 25 2009Jan 26 2009

Keywords

  • Cancer Detection
  • Fluorescence
  • Hyperspectral
  • Imaging
  • Oral Cancer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'In vivo fluorescence hyperspectral imaging of oral neoplasia'. Together they form a unique fingerprint.

Cite this