TY - GEN
T1 - In vivo imaging of radiopaque resorbable inferior vena cava filter infused with gold nanoparticles
AU - Tian, Li
AU - Lee, Patrick
AU - Singhana, Burapol
AU - Chen, Aaron
AU - Qiao, Yang
AU - Lu, Linfeng
AU - Martinez, Jonathan
AU - Tasciotti, Ennio
AU - Jacobsen, Megan C.
AU - Melancon, Adam
AU - McArthur, Mark
AU - Eggers, Mitch
AU - Huang, Steven
AU - Melancon, Marites P.
N1 - Publisher Copyright:
© 2018 SPIE.
PY - 2018
Y1 - 2018
N2 - Radiopaque resorbable inferior vena cava filter (IVCF) were developed to offer a less expensive alternative to assessing filter integrity in preventing pulmonary embolism for the recommended prophylactic period and then simply vanishes without intervention. In this study, we determined the efficacy of gold nanoparticle (AuNP)-infused poly-p-dioxanone (PPDO) as an IVCF in a swine model. Infusion into PPDO loaded 1.14±0.08 % AuNP by weight as determined by elemental analysis. The infusion did not alter PPDO's mechanical strength nor crystallinity (Kruskalâ'Wallis one-way ANOVA, p<0.05). There was no cytotoxicity observed (one-way ANOVA, p<0.05) when tested against RF24 and MRC5 cells. Gold content in PPDO was maintained at ∼2000 ppm during the 6-week incubation in PBS at 37oC. As a proof-of-concept, two pigs were deployed with IVCF, one with AuNP-PPDO and the other without coating. Results show that the stent ring of AuNP-PPDO was highly visible even in the presence of iodine-based contrast agent and after clot introduction, but not of the uncoated IVCF. Autopsy at two weeks post-implantation showed AuNP-PPDO filter was endothelialized onto the IVC wall, and no sign of filter migration was observed. The induced clot was also still trapped within the AuNP-PPDO IVCF. As a conclusion, we successfully fabricated AuNP-infused PPDO IVCF that is radiopaque, has robust mechanical strength, biocompatible, and can be imaged effectively in vivo. This suggests the efficacy of this novel, radiopaque, absorbable IVCF for monitoring its position and integrity over time, thus increasing the safety and efficacy of deep vein thrombosis treatment.
AB - Radiopaque resorbable inferior vena cava filter (IVCF) were developed to offer a less expensive alternative to assessing filter integrity in preventing pulmonary embolism for the recommended prophylactic period and then simply vanishes without intervention. In this study, we determined the efficacy of gold nanoparticle (AuNP)-infused poly-p-dioxanone (PPDO) as an IVCF in a swine model. Infusion into PPDO loaded 1.14±0.08 % AuNP by weight as determined by elemental analysis. The infusion did not alter PPDO's mechanical strength nor crystallinity (Kruskalâ'Wallis one-way ANOVA, p<0.05). There was no cytotoxicity observed (one-way ANOVA, p<0.05) when tested against RF24 and MRC5 cells. Gold content in PPDO was maintained at ∼2000 ppm during the 6-week incubation in PBS at 37oC. As a proof-of-concept, two pigs were deployed with IVCF, one with AuNP-PPDO and the other without coating. Results show that the stent ring of AuNP-PPDO was highly visible even in the presence of iodine-based contrast agent and after clot introduction, but not of the uncoated IVCF. Autopsy at two weeks post-implantation showed AuNP-PPDO filter was endothelialized onto the IVC wall, and no sign of filter migration was observed. The induced clot was also still trapped within the AuNP-PPDO IVCF. As a conclusion, we successfully fabricated AuNP-infused PPDO IVCF that is radiopaque, has robust mechanical strength, biocompatible, and can be imaged effectively in vivo. This suggests the efficacy of this novel, radiopaque, absorbable IVCF for monitoring its position and integrity over time, thus increasing the safety and efficacy of deep vein thrombosis treatment.
KW - Gold nanoparticles (AuNP)
KW - Inferior vena cava filter (IVCF)
KW - Poly-p-dioxanone (PPDO)
KW - Pulmonary embolism
KW - X-ray and computed tomography (CT)
UR - http://www.scopus.com/inward/record.url?scp=85050691782&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85050691782&partnerID=8YFLogxK
U2 - 10.1117/12.2293738
DO - 10.1117/12.2293738
M3 - Conference contribution
C2 - 31406393
AN - SCOPUS:85050691782
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Medical Imaging 2018
A2 - Fei, Baowei
A2 - Webster, Robert J.
PB - SPIE
T2 - Medical Imaging 2018: Image-Guided Procedures, Robotic Interventions, and Modeling
Y2 - 12 February 2018 through 15 February 2018
ER -