In vivo molecular imaging to diagnose and subtype tumors through receptor-targeted optically labeled monoclonal antibodies

Yoshinori Koyama, Tristan Barrett, Yukihiro Hama, Gregory Ravizzini, Peter L. Choyke, Hisataka Kobayashi

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Molecular imaging of cell surface receptors can potentially diagnose tumors based on their distinct expression profiles. Using multifilter spectrally resolved optical imaging with three fluorescently labeled antibodies, we simultaneously imaged three different cell surface receptors to distinguish tumor types non-invasively. We selected tumors overexpressing different subtypes of EGFR receptor: HER-1 (A431) and HER-2 (NIH3T3/HER2+), or interleukin-2 receptor α-subunit receptor (IL-2Rα; SP2/Tac). After tumor establishment, a cocktail of three fluorescently labeled monoclonal antibodies was injected: cetuximab-Cy5 (targeting HER-1), trastuzumab-Cy7(HER-2) ,and daclizumab-AlexaFluor-700 (IL-2Ra). Optical fluorescence imaging was performed after 24 hours with both a red filter set and three successive filter sets (yellow, red, and deep red). Spectrally resolved imaging of 10 mice clearly distinguished A431, NIH3T3/HER2+, and SP2-Tac tumors based on their distinct optical spectra. Three-filter sets significantly increased the signal-to-background ratio compared to a single-filter set by reducing the background signal, thus significantly improving the differentiation of each of the receptors targeted (P < .022). In conclusion, following multifilter spectrally resolved imaging, different tumor types can be simultaneously distinguished and diagnosed in vivo. Multiple filter sets increase the signal-to-noise ratio by substantially reducing the background signal, and may allow more optical dyes to be resolved within the narrow limits of the near-infrared spectrum.

Original languageEnglish (US)
Pages (from-to)1021-1029
Number of pages9
JournalNeoplasia
Volume9
Issue number12
DOIs
StatePublished - Dec 2007
Externally publishedYes

Keywords

  • Antibody cocktail
  • Contrast agent
  • Growth factor receptor
  • Near-infrared
  • Optical imaging

ASJC Scopus subject areas

  • Cancer Research

Fingerprint

Dive into the research topics of 'In vivo molecular imaging to diagnose and subtype tumors through receptor-targeted optically labeled monoclonal antibodies'. Together they form a unique fingerprint.

Cite this