Abstract
The dose dependence of near-infrared (NIR) fluorescent labeled RGD peptide targeted to the α vβ 3 integrin was assessed from xenografts bearing a subcutaneous human Kaposi's sarcoma (KS1767) with dynamic MR fluorescence optical imaging. The three-compartment pharmacokinetic (PK) model was used to determine PK parameters from fluorescence images acquired with an intensified charge-coupled device (ICCD) system. Dynamic imaging of Kaposi's sarcoma bearing animals was conducted with i.v. administration of Cy5.5-c(KRGDf) at doses of 0.75 to 6 nmol/animal and at the doses of 300 or 600 nmol of c(KRGDf) administered 1 hour before the injection of 3 nmol dose of the conjugate. The results show early and rapid uptake of Cy5.5-c(KRGDf), which was mediated by the administration of c(KRGDf) 1 hour before administration at the conjugate agent. From the results we found a linear increase in PK uptake rates at doses of 0.75 to 1.5 nmol, reflecting unsaturated binding to the integrin receptor. However, the results show the dose independence at large dose amounts from 3 to 6 nmol per animal. The effects of cancer treatments as well as diagnostics may be evaluated by in vivo PK analysis with NIR fluorescence optical imaging.
Original language | English (US) |
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Article number | 47 |
Pages (from-to) | 263-269 |
Number of pages | 7 |
Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 5689 |
DOIs | |
State | Published - 2005 |
Event | Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV - San Jose, CA, United States Duration: Jan 22 2005 → Jan 23 2005 |
Keywords
- Kaposi's sarcoma (KS1767)
- Near-infrared (NIR) fluorescence optical imaging
- Pharmacokinetic (PK)
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging