TY - JOUR
T1 - Increased blastogenic responses to worm antigen and loss of adherent suppressor cell activity after treatment for human infection with schistosoma mansoni
AU - Ellner, Jerrold J.
AU - Tweardy, David J.
AU - Osman, Gamal S.
AU - Wilson, Carla
AU - El Kholy, Aziz
AU - Rocklin, Ross E.
N1 - Funding Information:
Received for publication March 8, 1984, and in revised form July 13, 1984. This work was supported by grant no. AI-15351 from the U.S. Public Health Service and by grants from the Rockefeller Foundation and the Edna McConnell Clark Foundation. We thank Z. Tharwat for technical assistance and Cheryl Inman and Joyce Cairns for preparation of the manuscript. Please address requests for reprints to Dr. Jerrold J. Ellner, Department of Medicine, University Hospitals of Cleveland, Cleveland, Ohio 44106.
PY - 1985/2
Y1 - 1985/2
N2 - Fifteen Egyptian subjects infected with Schistosoma mansoni were evaluated parasitologically, clinically, and immunologically; treated with praziquantel; and reevaluated nine months later. Fecal egg counts were 97% lower after therapy; seven subjects no longer excreted eggs, as determined by Kato thick smears. Optimal [3H]thymidine incorporation induced by soluble adult worm antigenic preparation (SWAP) in peripheral blood mononuclear cells (PBMCs) increased significantly, from 4, 041 ± 434 (mean change in cpm ± SE) before treatment to 11, 232 ± 3, 414 after treatment (P <.0005). The relation between dose of antigen and response also shifted; the SWAP concentration producing optimal responses was 30.0 µg/ml before and 1.0 µg/ml after therapy. Before treatment, depletion of adherent cells from PBMCs in six subjects resulted in enhancement of responses to SWAP from 5, 575 ± 1, 210 to 14, 719 ± 8, 190 (P <.025). However, in these same individuals after treatment, PBMC and nonadherent lymphocyte responses were similar (22, 917 ± 6, 505 and 21, 239 ± 6, 122). These studies indicate loss of activity of adherent suppressor cells after treatment of chronic infection with S. mansoni. Waning of cellular regulatory mechanisms as the parasite load decreases may contribute to restoration of blastogenic responses.
AB - Fifteen Egyptian subjects infected with Schistosoma mansoni were evaluated parasitologically, clinically, and immunologically; treated with praziquantel; and reevaluated nine months later. Fecal egg counts were 97% lower after therapy; seven subjects no longer excreted eggs, as determined by Kato thick smears. Optimal [3H]thymidine incorporation induced by soluble adult worm antigenic preparation (SWAP) in peripheral blood mononuclear cells (PBMCs) increased significantly, from 4, 041 ± 434 (mean change in cpm ± SE) before treatment to 11, 232 ± 3, 414 after treatment (P <.0005). The relation between dose of antigen and response also shifted; the SWAP concentration producing optimal responses was 30.0 µg/ml before and 1.0 µg/ml after therapy. Before treatment, depletion of adherent cells from PBMCs in six subjects resulted in enhancement of responses to SWAP from 5, 575 ± 1, 210 to 14, 719 ± 8, 190 (P <.025). However, in these same individuals after treatment, PBMC and nonadherent lymphocyte responses were similar (22, 917 ± 6, 505 and 21, 239 ± 6, 122). These studies indicate loss of activity of adherent suppressor cells after treatment of chronic infection with S. mansoni. Waning of cellular regulatory mechanisms as the parasite load decreases may contribute to restoration of blastogenic responses.
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U2 - 10.1093/infdis/151.2.320
DO - 10.1093/infdis/151.2.320
M3 - Article
C2 - 3155782
AN - SCOPUS:0021989912
SN - 0022-1899
VL - 151
SP - 320
EP - 324
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -