Abstract
Spermidine/spermine N1-acetyltransferase (SSAT) regulates polyamine catabolism. Thioredoxin-1 (Trx-1) is a redox protein that is overexpressed in human cancer leading to increased cell proliferation, decreased apoptosis, and decreased patient survival. We report that SSAT mRNA expression is decreased in Trx-1 transfected MCF-7 human breast cancer cells. There is also a decrease in SSAT enzyme activity and lower putrescine levels but no change in spermine or spermidine levels. The expression of SSAT is regulated by the NF-E2-related factor 2 (Nrf-2) and polyamine modulated factor-1 (PMF-1) transcription factor complex. Trx-1 transfected MCF-7 cells showed decreased Nrf-2/PMF-1 DNA binding without a change in Nrf-2 or PMF-1 protein expression. The results suggest that Trx-1 may play a role in the redox regulation of SSAT expression and polyamine homeostasis that could contribute to the biological effects of Trx-1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 469-475 |
| Number of pages | 7 |
| Journal | Biochemical and biophysical research communications |
| Volume | 306 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 27 2003 |
Keywords
- Breast cancer
- Nrf-2/PMF-1
- Polyamines
- Putrescine
- Spermidine/spermine N-acetyltransferase
- Thioredoxin-1
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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