Influence of circulating antigen on blood pool activity of a radioiodinated monoclonal antibody

M. R. Zalutsky; R. C. Knapp; R. C. Bast

doi:10.1016/0883-2897(88)90014-1

Influence of circulating antigen on blood pool activity of a radioiodinated monoclonal antibody

M. R. Zalutsky
, R. C. Knapp
, R. C. Bast

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Athymic mice with and without circulating CA 125 antigen were injected with 0.1-100μg of ¹³¹I-labeled OC 125 F(ab′)₂ antibody fragment. Both the blood clearance of ¹³¹I activity and the change in serum CA 125 were monitored over 24 h. Influence of CA 125 on blood pool activity could be avoided only at the 100 βg dose. In patient studies, circulating CA 125 levels decreased for the first 2 h after injection of OC 125 F(ab′)₂ but generally returned to preinjection levels shortly thereafter. In vitro binding studies using the sera from patients injected with ¹³¹I-labeled OC 125 F(ab′)₂ suggest that circulating CA 125 could interfere with the tumor uptake of the labeled antibody.

Original language	English (US)
Pages (from-to)	431-437
Number of pages	7
Journal	International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
Volume	15
Issue number	4
DOIs	https://doi.org/10.1016/0883-2897(88)90014-1
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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10.1016/0883-2897(88)90014-1

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@article{21370bc3289447c0b5123e46a0601a87,

title = "Influence of circulating antigen on blood pool activity of a radioiodinated monoclonal antibody",

abstract = "Athymic mice with and without circulating CA 125 antigen were injected with 0.1-100μg of 131I-labeled OC 125 F(ab′)2 antibody fragment. Both the blood clearance of 131I activity and the change in serum CA 125 were monitored over 24 h. Influence of CA 125 on blood pool activity could be avoided only at the 100 βg dose. In patient studies, circulating CA 125 levels decreased for the first 2 h after injection of OC 125 F(ab′)2 but generally returned to preinjection levels shortly thereafter. In vitro binding studies using the sera from patients injected with 131I-labeled OC 125 F(ab′)2 suggest that circulating CA 125 could interfere with the tumor uptake of the labeled antibody.",

author = "Zalutsky, \{M. R.\} and Knapp, \{R. C.\} and Bast, \{R. C.\}",

note = "Funding Information: Supported in part by NC1 Grant Number CA 42324. *For reprints contact: Michael R. Zalutsky, Ph.D., Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, U.S.A.",

year = "1988",

doi = "10.1016/0883-2897(88)90014-1",

language = "English (US)",

volume = "15",

pages = "431--437",

journal = "International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology",

issn = "0883-2897",

publisher = "Elsevier BV",

number = "4",

}

TY - JOUR

T1 - Influence of circulating antigen on blood pool activity of a radioiodinated monoclonal antibody

AU - Zalutsky, M. R.

AU - Knapp, R. C.

AU - Bast, R. C.

N1 - Funding Information: Supported in part by NC1 Grant Number CA 42324. *For reprints contact: Michael R. Zalutsky, Ph.D., Department of Radiology, Duke University Medical Center, Box 3808, Durham, NC 27710, U.S.A.

PY - 1988

Y1 - 1988

N2 - Athymic mice with and without circulating CA 125 antigen were injected with 0.1-100μg of 131I-labeled OC 125 F(ab′)2 antibody fragment. Both the blood clearance of 131I activity and the change in serum CA 125 were monitored over 24 h. Influence of CA 125 on blood pool activity could be avoided only at the 100 βg dose. In patient studies, circulating CA 125 levels decreased for the first 2 h after injection of OC 125 F(ab′)2 but generally returned to preinjection levels shortly thereafter. In vitro binding studies using the sera from patients injected with 131I-labeled OC 125 F(ab′)2 suggest that circulating CA 125 could interfere with the tumor uptake of the labeled antibody.

AB - Athymic mice with and without circulating CA 125 antigen were injected with 0.1-100μg of 131I-labeled OC 125 F(ab′)2 antibody fragment. Both the blood clearance of 131I activity and the change in serum CA 125 were monitored over 24 h. Influence of CA 125 on blood pool activity could be avoided only at the 100 βg dose. In patient studies, circulating CA 125 levels decreased for the first 2 h after injection of OC 125 F(ab′)2 but generally returned to preinjection levels shortly thereafter. In vitro binding studies using the sera from patients injected with 131I-labeled OC 125 F(ab′)2 suggest that circulating CA 125 could interfere with the tumor uptake of the labeled antibody.

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UR - https://www.scopus.com/pages/publications/45549117751#tab=citedBy

U2 - 10.1016/0883-2897(88)90014-1

DO - 10.1016/0883-2897(88)90014-1

M3 - Article

C2 - 3255739

AN - SCOPUS:45549117751

SN - 0883-2897

VL - 15

SP - 431

EP - 437

JO - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology

JF - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology

IS - 4

ER -

Influence of circulating antigen on blood pool activity of a radioiodinated monoclonal antibody

Abstract

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