Influence of H-2K transfection on susceptibility of fibrosarcoma tumor cells to natural killer (NK) cells

Jacob Gopas; Shraga Segal; Gunter Hammerling; Menashe Bar-Eli; Bracha Rager-Zisman

doi:10.1016/0165-2478(88)90039-9

Influence of H-2K transfection on susceptibility of fibrosarcoma tumor cells to natural killer (NK) cells

Jacob Gopas
, Shraga Segal
, Gunter Hammerling
, Menashe Bar-Eli
, Bracha Rager-Zisman

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

In this study, we have used the T-10 fibrosarcoma tumor cells to further analyze the relationship between metastatic competence, expression of H-2K antigens and susceptibility to lysis by virus augmented NK cells in vitro. Our results show an inverse correlation between metastatic properties of the original T-10 clones, IC9 and IE7, and susceptibility in vitro to lysis by virus-augmented NK cells. Restoration by transfection of expression of H-2K genes (H-2K^b or H-2K^k) led to the alteration of the metastatic phenotype of the tumors cells, yet had minor influence on the putative susceptibility of these clones to NK. These observations suggest that expression of MHC gene products, while affecting metastases, does not exclusively determine sensitivity to NK cells.

Original language	English (US)
Pages (from-to)	261-265
Number of pages	5
Journal	Immunology Letters
Volume	17
Issue number	3
DOIs	https://doi.org/10.1016/0165-2478(88)90039-9
State	Published - Mar 1988

Keywords

H-2K transfection
Metastasis
Natural killer cells

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0165-2478(88)90039-9

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title = "Influence of H-2K transfection on susceptibility of fibrosarcoma tumor cells to natural killer (NK) cells",

abstract = "In this study, we have used the T-10 fibrosarcoma tumor cells to further analyze the relationship between metastatic competence, expression of H-2K antigens and susceptibility to lysis by virus augmented NK cells in vitro. Our results show an inverse correlation between metastatic properties of the original T-10 clones, IC9 and IE7, and susceptibility in vitro to lysis by virus-augmented NK cells. Restoration by transfection of expression of H-2K genes (H-2Kb or H-2Kk) led to the alteration of the metastatic phenotype of the tumors cells, yet had minor influence on the putative susceptibility of these clones to NK. These observations suggest that expression of MHC gene products, while affecting metastases, does not exclusively determine sensitivity to NK cells.",

keywords = "H-2K transfection, Metastasis, Natural killer cells",

author = "Jacob Gopas and Shraga Segal and Gunter Hammerling and Menashe Bar-Eli and Bracha Rager-Zisman",

note = "Funding Information: We thank Mrs. E Zuckerman and Mrs. Y. Segev for excellent technical assistance and Mrs. C. Simon for secretarial work in the preparation of this manuscript. B.R.Z. and J.G. were supported by a grant from the Israel Cancer Research Fund, U.S.A. and Canada and M.B. by DKFZ Grant No. Ca27, Heidelberg, Germany and the National Council for Research Development, Israel.",

year = "1988",

month = mar,

doi = "10.1016/0165-2478(88)90039-9",

language = "English (US)",

volume = "17",

pages = "261--265",

journal = "Immunology Letters",

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T1 - Influence of H-2K transfection on susceptibility of fibrosarcoma tumor cells to natural killer (NK) cells

AU - Gopas, Jacob

AU - Segal, Shraga

AU - Hammerling, Gunter

AU - Bar-Eli, Menashe

AU - Rager-Zisman, Bracha

N1 - Funding Information: We thank Mrs. E Zuckerman and Mrs. Y. Segev for excellent technical assistance and Mrs. C. Simon for secretarial work in the preparation of this manuscript. B.R.Z. and J.G. were supported by a grant from the Israel Cancer Research Fund, U.S.A. and Canada and M.B. by DKFZ Grant No. Ca27, Heidelberg, Germany and the National Council for Research Development, Israel.

PY - 1988/3

Y1 - 1988/3

N2 - In this study, we have used the T-10 fibrosarcoma tumor cells to further analyze the relationship between metastatic competence, expression of H-2K antigens and susceptibility to lysis by virus augmented NK cells in vitro. Our results show an inverse correlation between metastatic properties of the original T-10 clones, IC9 and IE7, and susceptibility in vitro to lysis by virus-augmented NK cells. Restoration by transfection of expression of H-2K genes (H-2Kb or H-2Kk) led to the alteration of the metastatic phenotype of the tumors cells, yet had minor influence on the putative susceptibility of these clones to NK. These observations suggest that expression of MHC gene products, while affecting metastases, does not exclusively determine sensitivity to NK cells.

AB - In this study, we have used the T-10 fibrosarcoma tumor cells to further analyze the relationship between metastatic competence, expression of H-2K antigens and susceptibility to lysis by virus augmented NK cells in vitro. Our results show an inverse correlation between metastatic properties of the original T-10 clones, IC9 and IE7, and susceptibility in vitro to lysis by virus-augmented NK cells. Restoration by transfection of expression of H-2K genes (H-2Kb or H-2Kk) led to the alteration of the metastatic phenotype of the tumors cells, yet had minor influence on the putative susceptibility of these clones to NK. These observations suggest that expression of MHC gene products, while affecting metastases, does not exclusively determine sensitivity to NK cells.

KW - H-2K transfection

KW - Metastasis

KW - Natural killer cells

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UR - https://www.scopus.com/pages/publications/0023914421#tab=citedBy

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DO - 10.1016/0165-2478(88)90039-9

M3 - Article

C2 - 2836303

AN - SCOPUS:0023914421

SN - 0165-2478

VL - 17

SP - 261

EP - 265

JO - Immunology Letters

JF - Immunology Letters

IS - 3

ER -

Influence of H-2K transfection on susceptibility of fibrosarcoma tumor cells to natural killer (NK) cells

Abstract

Keywords

ASJC Scopus subject areas

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