Integrating piggyBac transposon transgenes into mouse fibroblasts using chemical methods

Richard Behringer; Marina Gertsenstein; Kristina Vintersten Nagy; Andras Nagy

doi:10.1101/pdb.prot092619

Integrating piggyBac transposon transgenes into mouse fibroblasts using chemical methods

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Genetics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Mouse embryonic fibroblasts can be reprogrammed to ES cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. We found that FuGENE HD transfection reagent can be effective for mouse embryonic fibroblasts (MEFs) to generate induced pluripotent stem cells (iPSCs) with the piggyBac transposon transgenes. There are multitudes of cell transfection methods commercially available. Their efficiency, and thus their success, in inducing pluripotent cell types are cell-type-dependent.

Original language	English (US)
Pages (from-to)	834-836
Number of pages	3
Journal	Cold Spring Harbor protocols
Volume	2017
Issue number	10
DOIs	https://doi.org/10.1101/pdb.prot092619
State	Published - Oct 2017

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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abstract = "Mouse embryonic fibroblasts can be reprogrammed to ES cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. We found that FuGENE HD transfection reagent can be effective for mouse embryonic fibroblasts (MEFs) to generate induced pluripotent stem cells (iPSCs) with the piggyBac transposon transgenes. There are multitudes of cell transfection methods commercially available. Their efficiency, and thus their success, in inducing pluripotent cell types are cell-type-dependent.",

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Integrating piggyBac transposon transgenes into mouse fibroblasts using chemical methods

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