Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.

Original languageEnglish (US)
Pages (from-to)831-833
Number of pages3
JournalCold Spring Harbor protocols
Volume2017
Issue number10
DOIs
StatePublished - Oct 2017

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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