Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation

Richard Behringer; Marina Gertsenstein; Kristina Vintersten Nagy; Andras Nagy

doi:10.1101/pdb.prot092601

Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation

Richard Behringer
, Marina Gertsenstein
, Kristina Vintersten Nagy
, Andras Nagy

Genetics

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.

Original language	English (US)
Pages (from-to)	831-833
Number of pages	3
Journal	Cold Spring Harbor protocols
Volume	2017
Issue number	10
DOIs	https://doi.org/10.1101/pdb.prot092601
State	Published - Oct 2017

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1101/pdb.prot092601

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abstract = "Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors{\textemdash}OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.",

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AU - Behringer, Richard

AU - Gertsenstein, Marina

AU - Vintersten Nagy, Kristina

AU - Nagy, Andras

PY - 2017/10

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AB - Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription factors—OCT4, SOX2, KLF4, and c-MYC. The piggyBac transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. This protocol is designed for use with the Neon electroporation system. It can be adapted to other types of electroporation systems.

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Integrating piggyBac transposon transgenes into mouse fibroblasts by electroporation

Abstract

ASJC Scopus subject areas

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