TY - JOUR
T1 - Interaction of peanut agglutinin, a lectin specific for nonreducing terminal d-galactosyl residues, with embryonal carcinoma cells
AU - Reisner, Yair
AU - Gachelin, Gabriel
AU - Dubois, Philippe
AU - Nicolas, Jean François
AU - Sharon, Nathan
AU - Jacob, François
N1 - Funding Information:
Yair Reisner was a recipient of a short-term EMBO fellowship granted for the initiation of this work. Nathan Sharon is an established Investigator of the Chief Scientist Office, Israel Ministry of Health.
Funding Information:
This work has been aided by contracts from the Delegation Generale a la Recherche Scientifique et Technique, the Centre National de la Recherche Scienti-fique, the Andre Meyer Foundation, the National Institute of Health (CA 163551, and the Institut National de la Sante et de la Recherche Medicale (No. 76.4.311.AU).
PY - 1977/11
Y1 - 1977/11
N2 - Peanut agglutinin (PNA), a lectin specific for terminal d-galactosyl residues, was found to react with embryonal carcinoma cells, but not with their differentiated derivatives. Receptors for PNA were detectable at the surface of all cells of the quasinullipotent F9 line and on only 50% of the multipotent PCC3/A/1 line. The fraction of the PCC3/A/1 population which expresses the F9 antigen was found to be included in the subpopulation carrying the PNA receptors. PNA+ and PNA- subpopulations of PCC3/A/1 were separated by a PNA-mediated reversible agglutination of PNA+ cells with rabbit erythrocytes. These subpopulations were essentially F9+ and F9-, respectively.
AB - Peanut agglutinin (PNA), a lectin specific for terminal d-galactosyl residues, was found to react with embryonal carcinoma cells, but not with their differentiated derivatives. Receptors for PNA were detectable at the surface of all cells of the quasinullipotent F9 line and on only 50% of the multipotent PCC3/A/1 line. The fraction of the PCC3/A/1 population which expresses the F9 antigen was found to be included in the subpopulation carrying the PNA receptors. PNA+ and PNA- subpopulations of PCC3/A/1 were separated by a PNA-mediated reversible agglutination of PNA+ cells with rabbit erythrocytes. These subpopulations were essentially F9+ and F9-, respectively.
UR - http://www.scopus.com/inward/record.url?scp=84886633605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886633605&partnerID=8YFLogxK
U2 - 10.1016/0012-1606(77)90338-4
DO - 10.1016/0012-1606(77)90338-4
M3 - Article
C2 - 924023
AN - SCOPUS:84886633605
SN - 0012-1606
VL - 61
SP - 20
EP - 27
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -