Interactions between c-kit and stem cell factor are not required for B- cell development in vivo

Shunichi Takeda, Takeyuki Shimizu, Hans-Reimer Rodewald

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    33 Scopus citations

    Abstract

    The receptor-type tyrosine kinase, c-kit is expressed in hematopoietic stem cells (HSC), myeloid, and lymphoid precursors. In c-kit ligand-deficient mice, absolute numbers of HSC are mildly reduced suggesting that c-kit is not essential for HSC development. However, c-kit- HSC cannot form spleen colonies or reconstitute hematopoietic functions in lethally irradiated recipient mice. Based on in in vitro experiments, a critical role of c-kit in B-cell development was suggested. Here we have investigated the B-cell development of c-kit-null mutant (W/W) mice in vivo. Furthermore, day 13 fetal liver cells from wild type or W/W mice were transferred into immunodeficient RAG-2(-/-) mice. Surprisingly, transferred c-kit cells gave rise to all stages of immature B cells in the bone marrow and subsequently to mature conventional B2, as well as B1, type B cells in the recipients to the same extent as transferred wild type cells. Hence, in contrast to important roles of c-kit in the expansion of HSC and the generation of erythroid and myeloid lineages and T-cell precursors, c-kit HSC can colonize the recipient bone marrow and differentiate into B cells in the absence of c-kit.

    Original languageEnglish (US)
    Pages (from-to)518-525
    Number of pages8
    JournalBlood
    Volume89
    Issue number2
    StatePublished - Jan 15 1997

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    ASJC Scopus subject areas

    • Biochemistry
    • Immunology
    • Hematology
    • Cell Biology

    Cite this

    Takeda, S., Shimizu, T., & Rodewald, H-R. (1997). Interactions between c-kit and stem cell factor are not required for B- cell development in vivo. Blood, 89(2), 518-525.