Intergenic transcripts originating from a subclass of ribosomal DNA repeats silence ribosomal RNA genes in trans

Raffaella Santoro, Kerstin Maike Schmitz, Juan Sandoval, Ingrid Grummt

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Epigenetic silencing of a fraction of ribosomal DNA (rDNA) requires association of the nucleolar chromatin-remodelling complex NoRC to 150-250 nucleotide RNAs (pRNA) that originate from an RNA polymerase I promoter located in the intergenic spacer separating rDNA repeats. Here, we show that NoRC-associated pRNA is transcribed from a sub-fraction of hypomethylated rRNA genes during mid S phase, acting in trans to inherit DNA methylation and transcriptional repression of late-replicating silent rDNA copies. The results reveal variability between individual rDNA clusters with distinct functional consequences.

Original languageEnglish (US)
Pages (from-to)52-58
Number of pages7
JournalEMBO reports
Volume11
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Ribosomal RNA
Ribosomal DNA
rRNA Genes
Genes
Ribosomal Spacer DNA
RNA Polymerase I
Chromatin Assembly and Disassembly
DNA Methylation
S Phase
Epigenomics
Chromatin
Nucleotides
RNA

Keywords

  • Epigenetic silencing
  • NoRC
  • Non-coding RNA
  • RDNA
  • Spacer promoter

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Intergenic transcripts originating from a subclass of ribosomal DNA repeats silence ribosomal RNA genes in trans. / Santoro, Raffaella; Schmitz, Kerstin Maike; Sandoval, Juan; Grummt, Ingrid.

In: EMBO reports, Vol. 11, No. 1, 01.2010, p. 52-58.

Research output: Contribution to journalArticle

Santoro, Raffaella ; Schmitz, Kerstin Maike ; Sandoval, Juan ; Grummt, Ingrid. / Intergenic transcripts originating from a subclass of ribosomal DNA repeats silence ribosomal RNA genes in trans. In: EMBO reports. 2010 ; Vol. 11, No. 1. pp. 52-58.
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