Intermediates in plasmid pT181 DNA replication

Sadhan Majumder; Richard P. Novick

doi:10.1093/nar/16.7.2897

Intermediates in plasmid pT181 DNA replication

Sadhan Majumder
, Richard P. Novick

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali accomodate this apparent discrepancy.

Original language	English (US)
Pages (from-to)	2897-2912
Number of pages	16
Journal	Nucleic acids research
Volume	16
Issue number	7
DOIs	https://doi.org/10.1093/nar/16.7.2897
State	Published - Apr 11 1988
Externally published	Yes

ASJC Scopus subject areas

Genetics

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abstract = "Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali accomodate this apparent discrepancy.",

author = "Sadhan Majumder and Novick, \{Richard P.\}",

note = "Funding Information: ACKNOWLEDGEMENT : This work was supported by a grant from National Institutes of General Medical Sciences (GM 14 372) R.P.N.",

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AU - Novick, Richard P.

N1 - Funding Information: ACKNOWLEDGEMENT : This work was supported by a grant from National Institutes of General Medical Sciences (GM 14 372) R.P.N.

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Y1 - 1988/4/11

N2 - Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali accomodate this apparent discrepancy.

AB - Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali accomodate this apparent discrepancy.

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Intermediates in plasmid pT181 DNA replication

Abstract

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