TY - CHAP
T1 - Isolation and Maintenance of Tumor-Infiltrating Lymphocytes for Translational and Clinical Applications
T2 - Established Methods and New Developments
AU - Fulbright, Orenthial J.
AU - Forget, Marie Andrée
AU - Haymaker, Cara
AU - Bernatchez, Chantale
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022
Y1 - 2022
N2 - Adoptive cell transfer (ACT) of in vitro expanded tumor-infiltrating lymphocytes (TIL) for the treatment of patients with advanced stages of metastatic melanoma remains one of the most beneficial therapies eliciting long-lasting responses. Methods and protocols used to expand TIL have evolved over time, utilizing different culture devices and other tools, to streamline and maximize the end product in both numbers and quality. Summarized in this chapter are the latest protocols used in the TIL program at MDACC.
AB - Adoptive cell transfer (ACT) of in vitro expanded tumor-infiltrating lymphocytes (TIL) for the treatment of patients with advanced stages of metastatic melanoma remains one of the most beneficial therapies eliciting long-lasting responses. Methods and protocols used to expand TIL have evolved over time, utilizing different culture devices and other tools, to streamline and maximize the end product in both numbers and quality. Summarized in this chapter are the latest protocols used in the TIL program at MDACC.
KW - Adoptive cell therapy (ACT)
KW - Cell culture
KW - Cytotoxic T-lymphocytes
KW - Immunotherapy
KW - Rapid expansion protocol (REP)
KW - Tumor-infiltrating lymphocytes (TIL)
UR - https://www.scopus.com/pages/publications/85122453853
UR - https://www.scopus.com/pages/publications/85122453853#tab=citedBy
U2 - 10.1007/978-1-0716-2014-4_5
DO - 10.1007/978-1-0716-2014-4_5
M3 - Chapter
C2 - 34993939
AN - SCOPUS:85122453853
T3 - Methods in Molecular Biology
SP - 43
EP - 71
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -