Isolation of functionally active T cell receptor γδ-bearing lymphocytes from human peripheral blood

Vidar Bosnes, Ragnhild Halvorsen, Gustav Gaudernack, Erik Thorsby

    Research output: Contribution to journalArticle

    7 Scopus citations

    Abstract

    We have developed a simple method for isolation of functionally active T cell receptor (TCR)-γδ positive cells from human peripheral blood, using immunomagnetic separation techniques. After culture with feeder cells and interleukin-2, cells thus isolated showed a CD3+ TCR-αβ- phenotype, and stained with antibodies against the TCR-γδ complex. The TCR-γδ+ cells were functionally active, responding with DNA synthesis when stimulated via their CD3 and CD2 molecules in concert, or when interleukin-2 was added.

    Original languageEnglish (US)
    Pages (from-to)251-255
    Number of pages5
    JournalJournal of Immunological Methods
    Volume118
    Issue number2
    DOIs
    StatePublished - Mar 31 1989

    Keywords

    • Cell separation
    • Immunomagnetic separation
    • T cell receptor-γδ

    ASJC Scopus subject areas

    • Biotechnology
    • Immunology

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