TY - JOUR
T1 - Isolation of high-molecular-weight DNA from mouse yolk sacs and the like
AU - Behringer, Richard
AU - Gertsenstein, Marina
AU - Nagy, Kristina Vintersten
AU - Nagy, Andras
PY - 2019/1
Y1 - 2019/1
N2 - Often, genotyping of mouse embryos is required, and a small part, such as the yolk sac, can be used for this purpose. Here, DNA samples are prepared from extra-embryonic tissues by digestion with Proteinase K and subsequent extraction. The yolk sac of mid-gestation or later-stage embryos provides a sufficient amount of DNA for Southern analysis. Small tissues of a few hundred cells are used for genotyping early postimplantation-and preimplantation-stage embryos by polymerase chain reaction (PCR).
AB - Often, genotyping of mouse embryos is required, and a small part, such as the yolk sac, can be used for this purpose. Here, DNA samples are prepared from extra-embryonic tissues by digestion with Proteinase K and subsequent extraction. The yolk sac of mid-gestation or later-stage embryos provides a sufficient amount of DNA for Southern analysis. Small tissues of a few hundred cells are used for genotyping early postimplantation-and preimplantation-stage embryos by polymerase chain reaction (PCR).
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U2 - 10.1101/pdb.prot092726
DO - 10.1101/pdb.prot092726
M3 - Article
C2 - 30602554
AN - SCOPUS:85059496142
SN - 1940-3402
VL - 2019
SP - 85
EP - 86
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 1
ER -